2-Color IHC Kit for 2 Mouse Antibody on Human Tissue, Brown/Fast Red
In immunohistochemistry, the technique of double staining is widely employed to simultaneously detect and differentiate two distinct antigens within a single tissue sample. The 2-Color IF Kit is designed to use DAB (brown), Fast Red (red) dye effectively stain 2 different antigens on human tissue or cell samples when paired with user-supplied 2 mouse primary antibodies.
Information
Kit Contents
HRP-Polymer anti-Mouse (RTU)
AP-Polymer anti-Mouse (RTU)
DAB Substrate (RTU)
DAB Chromogen (20x)
Double Mouse Blocker 1 (RTU)
Double Mouse Blocker 2 (RTU)
Fast Red Tablet
Fast Red Substrate (RTU)
Mounting Medium (RTU)
AP-Polymer anti-Mouse (RTU)
DAB Substrate (RTU)
DAB Chromogen (20x)
Double Mouse Blocker 1 (RTU)
Double Mouse Blocker 2 (RTU)
Fast Red Tablet
Fast Red Substrate (RTU)
Mounting Medium (RTU)
Usage
The kit provides two polymer enzyme conjugates: HRP polymer anti-Mouse IgG and AP polymer anti-Mouse IgG. It includes two distinct substrates/chromogens: DAB (brown color, reacts with HRP polymer anti-Mouse IgG) and Fast Red (red color, reacts with AP polymer anti-Mouse IgG). To prevent non-specific binding caused by endogenous biotin, the kit utilizes a non-biotin system.
Applications
Paraffin tissue (verified), frozen specimen and freshly prepared monolayer cell smears.
Color
DAB (brown),Fastred (red)
Specificity
Mouse
Tissue Species
Human
Storage
2-8℃
Note
The outcome is significantly influenced by several factors, including fixation, tissue slide thickness, antigen retrieval, as well as the dilution and incubation time of the primary antibody. It is crucial for the investigator to carefully evaluate all of these variables and establish the ideal conditions in order to accurately interpret the results.