2-Color IHC Kit for 2 Mouse Antibody on Human Tissue, Brown/Red
In immunohistochemistry, the technique of double staining is widely employed to simultaneously detect and differentiate two distinct antigens within a single tissue sample. The 2-Color IF Kit is designed to use DAB (brown) and Permanent Red (red) dye effectively stain 2 different antigens on human tissue or cell samples when paired with user-supplied 2 mouse primary antibodies.
Information
Kit Contents
HRP-Polymer anti-Mouse (RTU)
AP-Polymer anti-Mouse (RTU)
DAB Substrate buffer (RTU)
DAB Chromogen (20x)
Antibody Blocker (40x)
Double Mouse Blocker 1 (RTU)
Double Mouse Blocker 2 (RTU)
Permanent Red Substrate (RTU)
Permanent Red Activator (5x)
Permanent Red Chromogen (100x)
Mounting Medium (RTU)
AP-Polymer anti-Mouse (RTU)
DAB Substrate buffer (RTU)
DAB Chromogen (20x)
Antibody Blocker (40x)
Double Mouse Blocker 1 (RTU)
Double Mouse Blocker 2 (RTU)
Permanent Red Substrate (RTU)
Permanent Red Activator (5x)
Permanent Red Chromogen (100x)
Mounting Medium (RTU)
Usage
The kit consists of two polymer enzyme conjugates: HRP polymer anti-Mouse IgG and AP polymer anti-Mouse IgG. It offers two different substrates/chromogens: DAB (brown color, to be used with HRP polymer anti-Mouse IgG) and Permanent Red (red color, to be used with AP polymer anti-Mouse IgG). To avoid endogenous biotin non-specific binding, the kit employs a non-biotin system.
Applications
Paraffin tissue (verified), frozen specimen and freshly prepared monolayer cell smears.
Color
DAB (brown) and Permanentred (red)
Specificity
Mouse
Tissue Species
Human
Storage
2-8℃
Note
The outcome is significantly influenced by several factors, including fixation, tissue slide thickness, antigen retrieval, as well as the dilution and incubation time of the primary antibody. It is crucial for the investigator to carefully evaluate all of these variables and establish the ideal conditions in order to accurately interpret the results.