2-Color IHC Kit for 2 Mouse Antibody on Human Tissue, Purple/Red
In immunohistochemistry, the technique of double staining is widely employed to simultaneously detect and differentiate two distinct antigens within a single tissue sample. The 2-Color IF Kit is designed to use BCIP/NBT (Purple) and AEC (Red) dye effectively stain 2 different antigens on human tissue or cell samples when paired with user-supplied 2 mouse primary antibodies.
Information
Kit Contents
HRP-Polymer anti-Mouse (RTU)
AP-Polymer anti-Mouse (RTU)
BCIP/NBT Solution (RTU)
Double Mouse Blocker 1 (RTU)
Double Mouse Blocker 2 (RTU)
AEC Substrate Buffer (20x)
AEC Chromogen (20x)
Hydrogen Peroxide (20x)
Mounting Medium (RTU)
AP-Polymer anti-Mouse (RTU)
BCIP/NBT Solution (RTU)
Double Mouse Blocker 1 (RTU)
Double Mouse Blocker 2 (RTU)
AEC Substrate Buffer (20x)
AEC Chromogen (20x)
Hydrogen Peroxide (20x)
Mounting Medium (RTU)
Usage
The kit includes two polymer enzyme conjugates: HRP polymer anti-Mouse IgG and AP polymer anti-Mouse IgG. It also provides two distinct substrates/chromogens: AEC (Red color, to be used with HRP polymer anti-Mouse IgG) and BCIP/NBT (Purple/Blue color, to be used with AP polymer anti-Mouse IgG). By incorporating a non-biotin system, the kit effectively prevents non-specific binding due to endogenous biotin.
Applications
Paraffin tissue (verified), frozen specimen and freshly prepared monolayer cell smears.
Color
BCIP/NBT (purple) and AEC (red)
Specificity
Mouse
Tissue Species
Human
Storage
2-8℃
Note
The outcome is significantly influenced by several factors, including fixation, tissue slide thickness, antigen retrieval, as well as the dilution and incubation time of the primary antibody. It is crucial for the investigator to carefully evaluate all of these variables and establish the ideal conditions in order to accurately interpret the results.