2-Color IHC Kit for 2 Mouse Antibody on Rodent Tissue, Brown/Fast Red
In immunohistochemistry, the technique of double staining is widely employed to simultaneously detect and differentiate two distinct antigens within a single tissue sample. The 2-Color IF Kit is designed to use DAB (brown), Fast Red (red) dye effectively stain 2 different antigens on mouse tissue or cell samples when paired with user-supplied 2 mouse primary antibodies.
Information
Kit Contents
Mouse Primer (RTU)
HRP-Polymer anti-Mouse (RTU)
AP-Polymer anti-Mouse (RTU)
DAB Substrate (RTU0
DAB Chromogen (20x)
Antibody Blocker (40x)
Double Mouse Blocker 1 (RTU)
Double Mouse Blocker 2 (RTU)
Fast Red Chromogen
Fast Red Substrate (RTU)
Mounting Medium (RTU)
HRP-Polymer anti-Mouse (RTU)
AP-Polymer anti-Mouse (RTU)
DAB Substrate (RTU0
DAB Chromogen (20x)
Antibody Blocker (40x)
Double Mouse Blocker 1 (RTU)
Double Mouse Blocker 2 (RTU)
Fast Red Chromogen
Fast Red Substrate (RTU)
Mounting Medium (RTU)
Usage
Inside the kit, you will find a primer system to enhance the activity of two polymer enzyme conjugates: anti-mouse IgG HRP-polymer and anti-mouse IgG AP-polymer. These conjugates are designed to work with two different substrates/chromogens: Fast Red and DAB.
Applications
Paraffin tissue (verified), frozen specimen and freshly prepared monolayer cell smears.
Color
DAB (brown),Fastred (red)
Specificity
Mouse
Tissue Species
Rodent
Storage
2-8℃
Note
The outcome is significantly influenced by several factors, including fixation, tissue slide thickness, antigen retrieval, as well as the dilution and incubation time of the primary antibody. It is crucial for the investigator to carefully evaluate all of these variables and establish the ideal conditions in order to accurately interpret the results.
Principle
Fast Red, in conjunction with anti-mouse IgG AP-polymer conjugate, generates a red color. DAB chromogen, in combination with anti-Mouse IgG HRP-polymer conjugate, produces a brown color. The kit utilizes a non-biotin system to avoid the extra steps involved in blocking non-specific binding due to endogenous biotin.