2-Color IHC Kit for 2 Mouse Antibody on Rodent Tissue, Brown/Red
In immunohistochemistry, the technique of double staining is widely employed to simultaneously detect and differentiate two distinct antigens within a single tissue sample. The 2-Color IF Kit is designed to use DAB (brown) and Permanent Red (red) dye effectively stain 2 different antigens on mouse tissue or cell samples when paired with user-supplied 2 mouse primary antibodies.
Information
Kit Contents
Mouse Primer (RTU)
HRP-Polymer anti-Mouse (RTU)
AP-Polymer anti-Mouse (RTU)
DAB Substrate (RTU)
DAB Chromogen (20x)
Antibody Blocker (40x)
Double Mouse Blocker 1 (RTU)
Double Mouse Blocker 2 (RTU)
Permanent Red Substrate (RTU)
Permanent Red Activator (5x)
Permanent Red Chromogen (100x)
Mounting Medium (RTU)
HRP-Polymer anti-Mouse (RTU)
AP-Polymer anti-Mouse (RTU)
DAB Substrate (RTU)
DAB Chromogen (20x)
Antibody Blocker (40x)
Double Mouse Blocker 1 (RTU)
Double Mouse Blocker 2 (RTU)
Permanent Red Substrate (RTU)
Permanent Red Activator (5x)
Permanent Red Chromogen (100x)
Mounting Medium (RTU)
Usage
The kit provides a primer system to enhance the activity of two polymer enzyme conjugates: anti-mouse IgG HRP-polymer and anti-mouse IgG AP polymer. These conjugates are accompanied by two distinct substrates/chromogens: Permanent Red and DAB.
Applications
Paraffin tissue (verified), frozen specimen and freshly prepared monolayer cell smears.
Color
DAB (brown) and Permanentred (red)
Specificity
Mouse
Tissue Species
Rodent
Storage
2-8℃
Note
The outcome is significantly influenced by several factors, including fixation, tissue slide thickness, antigen retrieval, as well as the dilution and incubation time of the primary antibody. It is crucial for the investigator to carefully evaluate all of these variables and establish the ideal conditions in order to accurately interpret the results.
Principle
Permanent Red, in conjunction with antimouse IgG AP-polymer conjugate, produces a red color. DAB chromogen, when combined with anti-mouse IgG HRP-polymer conjugate, results in a brown color. The kit employs a non-biotin system to avoid the additional steps required for blocking non-specific binding caused by endogenous biotin.