2-Color IHC Kit for 2 Mouse Antibody on Rodent Tissue, Geen/Red
In immunohistochemistry, the technique of double staining is widely employed to simultaneously detect and differentiate two distinct antigens within a single tissue sample. The 2-Color IF Kit is designed to use Emerald (Green) and Permanent Red (Red) dye effectively stain 2 different antigens on mouse tissue or cell samples when paired with user-supplied 2 mouse primary antibodies.
Information
Kit Contents
Mouse Primer (RTU)
HRP-Polymer anti-Mouse (RTU)
AP-Polymer anti-Mouse (RTU)
Permanent Red Substrate (RTU)
Permanent Red Activator (5x）
Permanent Red Chromogen (100x)
Antibody Blocker (40x)
Double Mouse Blocker 1 (RTU)
Double Mouse Blocker 2 (RTU)
Emerald Chromogen (RTU)
Mounting Medium (RTU)
HRP-Polymer anti-Mouse (RTU)
AP-Polymer anti-Mouse (RTU)
Permanent Red Substrate (RTU)
Permanent Red Activator (5x）
Permanent Red Chromogen (100x)
Antibody Blocker (40x)
Double Mouse Blocker 1 (RTU)
Double Mouse Blocker 2 (RTU)
Emerald Chromogen (RTU)
Mounting Medium (RTU)
Usage
Included in the kit is a primer system to enhance the activity of two polymer enzyme conjugates: anti-mouse IgG HRP-polymer and anti-mouse IgG AP-polymer. These conjugates are designed to work with two distinct substrates/chromogen: Permanent Red and Emerald.
Applications
Paraffin tissue (verified), frozen specimen and freshly prepared monolayer cell smears.
Color
Emerald (green) and Permanentred (red)
Specificity
Mouse
Tissue Species
Rodent
Storage
2-8℃
Note
The outcome is significantly influenced by several factors, including fixation, tissue slide thickness, antigen retrieval, as well as the dilution and incubation time of the primary antibody. It is crucial for the investigator to carefully evaluate all of these variables and establish the ideal conditions in order to accurately interpret the results.
Principle
Permanent Red, in conjunction with anti-mouse IgG AP-polymer conjugate, produces a red color. Emerald chromogen, in combination with anti-Mouse IgG HRP-polymer conjugate, generates a green color. However, when both chromogens are produced in the same location, the resulting color appears as blue to purple. The kit utilizes a non-biotin system to prevent non-specific binding of endogenous biotin and avoids the extra steps involved in blocking such binding.