2-Color IHC Kit for 2 Mouse Antibody on Rodent Tissue, Purple/Red
In immunohistochemistry, the technique of double staining is widely employed to simultaneously detect and differentiate two distinct antigens within a single tissue sample. The 2-Color IF Kit is designed to use BCIP/NBT (Purple) and AEC (Red) dye effectively stain 2 different antigens on mouse tissue or cell samples when paired with user-supplied 2 mouse primary antibodies.
Information
Kit Contents
Mouse AP Polymer (RTU)
BCIP/NBT (RTU)
DAB Substrate(RTU)
Double Mouse Blocker 1 (RTU)
Double Mouse Blocker 2 (RTU)
Mouse Primer (RTU)
Mouse HRP(AEC) Polymer (RTU)
AEC Substrate (20x)
AEC Chromogen (20x)
Hydrogen Peroxide (20x)
Mounting Medium (RTU)
BCIP/NBT (RTU)
DAB Substrate(RTU)
Double Mouse Blocker 1 (RTU)
Double Mouse Blocker 2 (RTU)
Mouse Primer (RTU)
Mouse HRP(AEC) Polymer (RTU)
AEC Substrate (20x)
AEC Chromogen (20x)
Hydrogen Peroxide (20x)
Mounting Medium (RTU)
Usage
The kit supplies a primer system to enhance the activity of two polymer enzyme conjugates: anti-mouse IgG HRP polymer and anti-mouse IgG AP polymer. These conjugates are accompanied by a specific substrates/chromogen: GBIAEC and BCIP/NBT.
Applications
Paraffin tissue (verified), frozen specimen and freshly prepared monolayer cell smears.
Color
BCIP/NBT (purple) and AEC (red)
Specificity
Mouse
Tissue Species
Rodent
Storage
2-8℃
Note
The outcome is significantly influenced by several factors, including fixation, tissue slide thickness, antigen retrieval, as well as the dilution and incubation time of the primary antibody. It is crucial for the investigator to carefully evaluate all of these variables and establish the ideal conditions in order to accurately interpret the results.
Principle
BCIP/NBT, in conjunction with anti-mouse IgG AP polymer conjugate, yields a purple color. AEC chromogen, when combined with anti-mouse IgG HRP-polymer conjugate, produces a red color. The kit employs a non-biotin system to avoid the additional steps required for blocking non-specific binding caused by endogenous biotin.