2-Color IHC Kit for 2 Rabbit Antibody on Human/Rodent Tissue, Brown/Red
In immunohistochemistry, the technique of double staining is widely employed to simultaneously detect and differentiate two distinct antigens within a single tissue sample. The 2-Color IF Kit is designed to use DAB (brown) and Permanent Red (red) dye effectively stain 2 different antigens on human and mouse tissue or cell samples when paired with user-supplied 2 rabbit primary antibodies.
Information
Kit Contents
HRP-Polymer anti-Rabbit (RTU）
AP-Polymer anti-Rabbit (RTU）
DAB Substrate (RTU)
DAB Chromogen (20X)
Double Rabbit Blocker 1 (RTU)
Double Rabbit Blocker 2 (RTU)
Permanent Red Substrate (RTU)
Permanent Red Activator (5x)
Permanent Red Chromogen (100x)
Mounting Medium (RTU)
AP-Polymer anti-Rabbit (RTU）
DAB Substrate (RTU)
DAB Chromogen (20X)
Double Rabbit Blocker 1 (RTU)
Double Rabbit Blocker 2 (RTU)
Permanent Red Substrate (RTU)
Permanent Red Activator (5x)
Permanent Red Chromogen (100x)
Mounting Medium (RTU)
Usage
The kit offers two polymer enzyme conjugates: HRP polymer anti-Rabbit IgG and AP polymer anti-Rabbit IgG. These conjugates are accompanied by two distinct substrates/chromogens: DAB (brown color, used with HRP polymer anti-Rabbit IgG) and Permanent Red (red color, used with AP polymer anti-Rabbit IgG).
Applications
Paraffin tissue (verified), frozen specimen and freshly prepared monolayer cell smears.
Color
DAB (brown) and Permanentred (red)
Specificity
Rabbit
Tissue Species
Human and Rodent
Storage
2-8℃
Note
The outcome is significantly influenced by several factors, including fixation, tissue slide thickness, antigen retrieval, as well as the dilution and incubation time of the primary antibody. It is crucial for the investigator to carefully evaluate all of these variables and establish the ideal conditions in order to accurately interpret the results.
Principle
When the AP Polymer anti-Rabbit antigen is present, only Permanent Red will be detected. Similarly, when HRP Polymer anti-rabbit antigen is present, only DAB (brown) will be detected. The kit employs a non-biotin system to avoid non-specific binding of endogenous biotin.