2-Color IHC Kit for 2 Rabbit Antibody on Human/Rodent Tissue, Geen/Red
In immunohistochemistry, the technique of double staining is widely employed to simultaneously detect and differentiate two distinct antigens within a single tissue sample. The 2-Color IF Kit is designed to use Emerald (Green) and Permanent Red (Red) dye effectively stain 2 different antigens on human and mouse tissue or cell samples when paired with user-supplied 2 rabbit primary antibodies.
Information
Kit Contents
HRP-Polymer anti-Rabbit (RTU）
AP-Polymer anti-Rabbit (RTU）
Permanent Red AP Substrate(RTU)
Permanent Red Activator (5x)
Permanent Red Chromogen(100x)
Double Rabbit Blocker 1 (RTU)
Double Rabbit Blocker 2 (RTU)
Emerald Chromogen (RTU)
Mounting Medium (RTU)
AP-Polymer anti-Rabbit (RTU）
Permanent Red AP Substrate(RTU)
Permanent Red Activator (5x)
Permanent Red Chromogen(100x)
Double Rabbit Blocker 1 (RTU)
Double Rabbit Blocker 2 (RTU)
Emerald Chromogen (RTU)
Mounting Medium (RTU)
Usage
The kit provides two polymer enzyme conjugates: HRP Polymer anti-Rabbit IgG and AP Polymer anti-Rabbit IgG. These conjugates are paired with two distinct substrates/chromogens: Permanent Red (red color, used with AP polymer anti-Rabbit IgG) and Emerald chromogen (green color, used with HRP polymer anti-Rabbit IgG).
Applications
Paraffin tissue (verified), frozen specimen and freshly prepared monolayer cell smears.
Color
Emerald (green) and Permanentred (red)
Specificity
Rabbit
Tissue Species
Human and Rodent
Storage
2-8℃
Note
The outcome is significantly influenced by several factors, including fixation, tissue slide thickness, antigen retrieval, as well as the dilution and incubation time of the primary antibody. It is crucial for the investigator to carefully evaluate all of these variables and establish the ideal conditions in order to accurately interpret the results.
Principle
If the co-localization area stains blue, it indicates a higher concentration of the antigen indicated by Emerald in the cell. If the color is purple, it signifies a higher concentration of the antigen indicated by Permanent-Red. The kit utilizes a non-biotin system to prevent non-specific binding of endogenous biotin.