2-Color IHC Kit for Goat/Mouse Antibody on Human Tissue, Brown/Red
In immunohistochemistry, the technique of double staining is widely employed to simultaneously detect and differentiate two distinct antigens within a single tissue sample. The 2-Color IF Kit is designed to use DAB (brown) and Permanent Red (red) dye effectively stain 2 different antigens on human tissue or cell samples when paired with user-supplied mouse and goat primary antibodies.
Information
Kit Contents
HRP-Polymer anti-Mouse (RTU)
AP-Polymer anti-Goat (RTU)
Permanent Red Substrate (RTU)
Permanent Red Activator (5x)
Permanent Red Chromogen (100x)
Double Stain Blocker
DAB Substrate(RTU)
DAB Chromogen(20x)
Mounting Medium (RTU)
AP-Polymer anti-Goat (RTU)
Permanent Red Substrate (RTU)
Permanent Red Activator (5x)
Permanent Red Chromogen (100x)
Double Stain Blocker
DAB Substrate(RTU)
DAB Chromogen(20x)
Mounting Medium (RTU)
Usage
The kit consists of two polymer enzyme conjugates: AP polymer anti-Goat IgG and HRP polymer anti-Mouse IgG. It offers two different substrates/chromogens: DAB (Brown) and Permanent Red (Red).
Applications
Paraffin tissue (verified), frozen specimen and freshly prepared monolayer cell smears.
Color
DAB (brown) and Permanentred (red)
Specificity
Goat and Mouse
Tissue Species
Human
Storage
2-8℃
Note
The outcome is significantly influenced by several factors, including fixation, tissue slide thickness, antigen retrieval, as well as the dilution and incubation time of the primary antibody. It is crucial for the investigator to carefully evaluate all of these variables and establish the ideal conditions in order to accurately interpret the results.
Principle
This simplified protocol offers convenience by sequentially applying the enzyme conjugates to the specimen. When the anti-goat antigen is present, only the Permanent Red chromogen will be visible. Alternatively, if the anti-mouse antigen is present, only the DAB (brown) chromogen will be detected. The kit employs a non-biotin system to prevent nonspecific binding of endogenous biotin.