2-Color IHC Kit for Goat/Mouse Antibody on Human Tissue, Purple/Red
In immunohistochemistry, the technique of double staining is widely employed to simultaneously detect and differentiate two distinct antigens within a single tissue sample. The 2-Color IF Kit is designed to use BCIP/NBT (Purple) and AEC (Red) dye effectively stain 2 different antigens on human tissue or cell samples when paired with user-supplied mouse and goat primary antibodies.
Information
Kit Contents
HRP-Polymer anti-Mouse (RTU)
AP-Polymer anti-Goat (RTU)
BCIP/NBT (RTU)
AEC Substrate (20x)
AEC Chromogen (20x)
Double Stain Blocker
Hydrogen Peroxide (20x)
Mounting Medium (RTU)
AP-Polymer anti-Goat (RTU)
BCIP/NBT (RTU)
AEC Substrate (20x)
AEC Chromogen (20x)
Double Stain Blocker
Hydrogen Peroxide (20x)
Mounting Medium (RTU)
Usage
The kit includes two polymer enzyme conjugates: AP polymer anti-Goat IgG and HRP (AEC) polymer anti-Mouse IgG. It provides two distinct substrates/chromogens: BCIP/NBT (Purple) and AEC (Red).
Applications
Paraffin tissue (verified), frozen specimen and freshly prepared monolayer cell smears.
Color
BCIP/NBT (purple) and AEC (red)
Specificity
Goat and Mouse
Tissue Species
Human
Storage
2-8℃
Note
The outcome is significantly influenced by several factors, including fixation, tissue slide thickness, antigen retrieval, as well as the dilution and incubation time of the primary antibody. It is crucial for the investigator to carefully evaluate all of these variables and establish the ideal conditions in order to accurately interpret the results.
Principle
To perform this procedure, the user will sequentially apply two enzyme conjugates onto the specimen. If only the anti-goat antigen is present, the BCIP/NBT (Purple) chromogen will be visible. Similarly, if only the mouse antigen is present, the AEC (Red) chromogen will be observed. This kit employs a nonbiotin system to prevent nonspecific binding of endogenous biotin.