2-Color IHC Kit for Goat/Rabbit Antibody on Human/Rodent Tissue, Geen/Red
In immunohistochemistry, the technique of double staining is widely employed to simultaneously detect and differentiate two distinct antigens within a single tissue sample. The 2-Color IF Kit is designed to use Emerald (Green) and Permanent Red (Red) dye effectively stain 2 different antigens on human and mouse tissue or cell samples when paired with user-supplied goat and rabbit primary antibodies.
Information
Kit Contents
HRP-Polymer anti-Goat (RTU）
AP-Polymer anti-Rabbit (RTU）
Permanent Red Substrate (RTU)
Permanent Red Activator (5x)
Permanent Red Chromogen (100x)
Emerald Chromogen (RTU)
Mounting Medium (RTU)
AP-Polymer anti-Rabbit (RTU）
Permanent Red Substrate (RTU)
Permanent Red Activator (5x)
Permanent Red Chromogen (100x)
Emerald Chromogen (RTU)
Mounting Medium (RTU)
Usage
The kit offers two polymer enzyme conjugates: HRP Polymer anti-Goat IgG and AP Polymer anti-Rabbit IgG. These conjugates are accompanied by two substrates/chromogens: Emerald (green color) and Permanent Red (red color).
Applications
Paraffin tissue (verified), frozen specimen and freshly prepared monolayer cell smears.
Color
Emerald (green) and Permanentred (red)
Specificity
Goat and Rabbit
Tissue Species
Human and Rodent
Storage
2-8℃
Note
The outcome is significantly influenced by several factors, including fixation, tissue slide thickness, antigen retrieval, as well as the dilution and incubation time of the primary antibody. It is crucial for the investigator to carefully evaluate all of these variables and establish the ideal conditions in order to accurately interpret the results.
Principle
Permanent Red, when combined with anti-rabbit AP polymer conjugate, produces a red color. Emerald chromogen, on the other hand, reacts with anti-Goat HRP polymer conjugate to generate a green color. When two proteins are co-expressed in the same location, the area of colocalization appears blue if Emerald is more abundant, and purple-blue if Permanent Red is more abundant. The kit employs a non-biotin system, eliminating the need for blocking steps to prevent non-specific binding of endogenous biotin.