2-Color IHC Kit for Goat/Rat Antibody on Human/Mouse Tissue, Purple/Red
In immunohistochemistry, the technique of double staining is widely employed to simultaneously detect and differentiate two distinct antigens within a single tissue sample. The 2-Color IF Kit is designed to use BCIP/NBT (Purple) and AEC (Red) dye effectively stain 2 different antigens on human and mouse tissue or cell samples when paired with user-supplied goat and rat primary antibodies.
Information
Kit Contents
HRP-Polymer anti-Goat (RTU）
AP-Polymer anti-Rat (RTU）
AEC Substrate (20x)
AEC Chromogen (20x)
Hydrogen Peroxide (20x)
Double Stain Blocker
Rat Primer (RTU)
BCIP/NBT (RTU)
Mounting Medium (RTU)
AP-Polymer anti-Rat (RTU）
AEC Substrate (20x)
AEC Chromogen (20x)
Hydrogen Peroxide (20x)
Double Stain Blocker
Rat Primer (RTU)
BCIP/NBT (RTU)
Mounting Medium (RTU)
Usage
The kit provides two polymer enzyme conjugates: HRP polymer anti-Goat IgG and AP polymer anti-Rat IgG. These conjugates are paired with two distinct substrates/chromogens: AEC (red color) and BCIP/NBT (purple color).
Applications
Paraffin tissue (verified), frozen specimen and freshly prepared monolayer cell smears.
Color
BCIP/NBT (purple) and AEC (red)
Specificity
Goat and Rat
Tissue Species
Human and Mouse
Storage
2-8℃
Note
The outcome is significantly influenced by several factors, including fixation, tissue slide thickness, antigen retrieval, as well as the dilution and incubation time of the primary antibody. It is crucial for the investigator to carefully evaluate all of these variables and establish the ideal conditions in order to accurately interpret the results.
Principle
The user will sequentially apply two enzyme conjugates onto the specimen. When both proteins are present, a distinct purple/red color will develop, depending on the presence and location of the antigens. If only the anti-goat antigen is present, only the AEC chromogen will be detected. Similarly, if the anti-Rat antigen is present, only the BCIP/NBT chromogen will be observed. The kit is a non-biotin system that prevents non-specific binding of endogenous biotin.