2-Color IHC Kit for Mouse/Rabbit Antibody on Human Tissue, Brown/Fast Red, V 1
In immunohistochemistry, the technique of double staining is widely employed to simultaneously detect and differentiate two distinct antigens within a single tissue sample. The 2-Color IF Kit is designed to use DAB (brown), Fast Red (red) dye effectively stain 2 different antigens on human tissue or cell samples when paired with user-supplied mouse and rabbit primary antibodies.
Information
Kit Contents
HRP-Polymer anti-Mouse (RTU)
AP-Polymer anti-Rabbit (RTU)
DAB substrate buffer (RTU)
DAB chromogen (20x)
Fast Red chromogen tablets
Fast Red substrate buffer (RTU)
Mounting Medium (RTU)
AP-Polymer anti-Rabbit (RTU)
DAB substrate buffer (RTU)
DAB chromogen (20x)
Fast Red chromogen tablets
Fast Red substrate buffer (RTU)
Mounting Medium (RTU)
Usage
The kit provides two polymer enzyme conjugates: HRP Polymer anti-Mouse IgG and AP Polymer anti-Rabbit IgG, along with two distinct substrates/chromogens. The DAB chromogen (brown color) is used with the HRP Polymer anti-Mouse IgG, and the Fast Red chromogen (red color) is used with the AP Polymer anti-Rabbit IgG.
Applications
Paraffin tissue (verified), frozen specimen and freshly prepared monolayer cell smears.
Color
DAB (brown),Fastred (red)
Specificity
Mouse and Rabbit
Tissue Species
Human
Storage
2-8℃
Note
The outcome is significantly influenced by several factors, including fixation, tissue slide thickness, antigen retrieval, as well as the dilution and incubation time of the primary antibody. It is crucial for the investigator to carefully evaluate all of these variables and establish the ideal conditions in order to accurately interpret the results.
Principle
Users have the flexibility to apply both enzyme conjugates simultaneously onto the specimen and mix them on the slide. This simplified approach offers a much faster and quicker protocol compared to a sequential procedure. Moreover, the kit utilizes a non-biotin system, eliminating the risk of non-specific binding caused by endogenous biotin.
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