2-Color IHC Kit for Mouse/Rabbit Antibody on Human Tissue, Brown/Fast Red, V 3
In immunohistochemistry, the technique of double staining is widely employed to simultaneously detect and differentiate two distinct antigens within a single tissue sample. The 2-Color IF Kit is designed to use DAB (brown), Fast Red (red) dye effectively stain 2 different antigens on human tissue or cell samples when paired with user-supplied mouse and rabbit primary antibodies.
Information
Kit Contents
HRP-Polymer anti-Rabbit (RTU)
AP-Polymer anti-Mouse (RTU)
DAB Substrate Buffer (RTU)
DAB Chromogen(20x)
Fast Red Tablet
Fast Red Substrate(RTU)
Mounting Medium (RTU)
AP-Polymer anti-Mouse (RTU)
DAB Substrate Buffer (RTU)
DAB Chromogen(20x)
Fast Red Tablet
Fast Red Substrate(RTU)
Mounting Medium (RTU)
Usage
The kit provides two polymer enzyme conjugates: HRP Polymer anti-Rabbit IgG and AP Polymer anti-Mouse IgG. It includes two distinct substrates/chromogens: DAB (brown color, used with HRP Polymer anti-Rabbit IgG) and Fast Red (red color, used with AP Polymer anti-Mouse IgG).
Applications
Paraffin tissue (verified), frozen specimen and freshly prepared monolayer cell smears.
Color
DAB (brown),Fastred (red)
Specificity
Mouse and Rabbit
Tissue Species
Human
Storage
4-8℃
Note
The outcome is significantly influenced by several factors, including fixation, tissue slide thickness, antigen retrieval, as well as the dilution and incubation time of the primary antibody. It is crucial for the investigator to carefully evaluate all of these variables and establish the ideal conditions in order to accurately interpret the results.
Principle
With this protocol, users have the option to apply both enzyme conjugates onto the specimen at the same time and mix them on the slide, resulting in a faster and more efficient procedure compared to a sequential method. The kit utilizes a non-biotin system, effectively preventing non-specific binding caused by endogenous biotin.
Related Products
- 2-Color IHC Kit for Mouse/Rabbit Antibody on Human Tissue, Brown/Fast Red, V 1
- 2-Color IHC Kit for Mouse/Rabbit Antibody on Human Tissue, Geen/Red
- 2-Color IHC Kit for Mouse/Rabbit Antibody on Human Tissue, Purple/Red
- 2-Color IHC Kit for Mouse/Rabbit Antibody on Human Tissue, Brown/Red
- 2-Color IHC Kit for Mouse/Rabbit Antibody on Human Tissue, Brown/Fast Red, V 2