2-Color IHC Kit for Mouse/Rabbit Antibody on Human Tissue, Brown/Red
In immunohistochemistry, the technique of double staining is widely employed to simultaneously detect and differentiate two distinct antigens within a single tissue sample. The 2-Color IF Kit is designed to use DAB (brown), Permanent Red (red) dye effectively stain 2 different antigens on human tissue or cell samples when paired with user-supplied mouse and rabbit primary antibodies.
Information
Kit Contents
HRP-Polymer anti-Mouse (RTU)
AP-Polymer anti-Rabbit (RTU)
DAB Substrate (RTU)
DAB Chromogen (20x)
Permanent Red Substrate (RTU)
Permanent Red Activator (5x)
Permanent Red Chromogen (100x)
Mounting Medium (RTU)
AP-Polymer anti-Rabbit (RTU)
DAB Substrate (RTU)
DAB Chromogen (20x)
Permanent Red Substrate (RTU)
Permanent Red Activator (5x)
Permanent Red Chromogen (100x)
Mounting Medium (RTU)
Usage
The kit contains an HRP-Polymer anti-Mouse IgG, an AP-Polymer anti-Rabbit IgG, and two distinct chromogens. The HRPPolymer anti-Mouse IgG is used with the DAB chromogen (brown color), and the AP-Polymer anti-Rabbit IgG is used with the GBI-Permanent Red chromogen (red color).
Applications
Paraffin tissue (verified), frozen specimen and freshly prepared monolayer cell smears.
Color
DAB (brown),Permanentred (red)
Specificity
Mouse and Rabbit
Tissue Species
Human
Storage
2-8℃
Note
The outcome is significantly influenced by several factors, including fixation, tissue slide thickness, antigen retrieval, as well as the dilution and incubation time of the primary antibody. It is crucial for the investigator to carefully evaluate all of these variables and establish the ideal conditions in order to accurately interpret the results.
Principle
The protocol offers simplified steps that result in a much faster process by applying the enzyme conjugates as a mixture to the specimen. Furthermore, the kit utilizes a non-biotin system to prevent non-specific binding caused by endogenous biotin.
Related Products
- 2-Color IHC Kit for Mouse/Rabbit Antibody on Human Tissue, Brown/Fast Red, V 3
- 2-Color IHC Kit for Mouse/Rabbit Antibody on Human Tissue, Brown/Fast Red, V 1
- 2-Color IHC Kit for Mouse/Rabbit Antibody on Human Tissue, Brown/Fast Red, V 2
- 2-Color IHC Kit for Mouse/Rabbit Antibody on Human Tissue, Geen/Red
- 2-Color IHC Kit for Mouse/Rabbit Antibody on Human Tissue, Purple/Red