2-Color IHC Kit for Mouse/Rabbit Antibody on Rodent Tissue, Brown/Red
In immunohistochemistry, the technique of double staining is widely employed to simultaneously detect and differentiate two distinct antigens within a single tissue sample. The 2-Color IF Kit is designed to use DAB (brown) and Permanent Red (red) dye effectively stain 2 different antigens on mouse tissue or cell samples when paired with user-supplied mouse and rabbit primary antibodies.
Information
Kit Contents
Mouse Primer (RTU)
HRP-Polymer anti-Mouse (RTU)
AP-Polymer anti-Rabbit (RTU)
DAB Substrate (RTU)
DAB Chromogen (20x)
Permanent Red Substrate (RTU)
Permanent Red Activator (5x)
Permanent Red Chromogen (100x)
Mounting Medium (RTU)
HRP-Polymer anti-Mouse (RTU)
AP-Polymer anti-Rabbit (RTU)
DAB Substrate (RTU)
DAB Chromogen (20x)
Permanent Red Substrate (RTU)
Permanent Red Activator (5x)
Permanent Red Chromogen (100x)
Mounting Medium (RTU)
Usage
The kit includes two polymer enzyme conjugates: Mouse HRP Polymer and Rabbit AP Polymer. These conjugates come with two different substrates/chromogens. The Mouse HRP Polymer utilizes DAB, generating a brown color, while the Rabbit AP Polymer uses Permanent Red, which produces a red color.
Applications
Paraffin tissue (verified), frozen specimen and freshly prepared monolayer cell smears.
Color
DAB (brown) and Permanentred (red)
Specificity
Mouse and Rabbit
Tissue Species
Rodent
Storage
2-8℃
Note
The outcome is significantly influenced by several factors, including fixation, tissue slide thickness, antigen retrieval, as well as the dilution and incubation time of the primary antibody. It is crucial for the investigator to carefully evaluate all of these variables and establish the ideal conditions in order to accurately interpret the results.
Principle
To improve the specificity of antibody staining, a primer step is incorporated. Both enzyme conjugates are applied simultaneously to the specimen and mixed on the slide. This kit offers a simplified protocol that is faster and easier compared to a sequential procedure. It employs a non-biotin system to prevent non-specific binding of endogenous biotin.