2-Color IHC Kit for Mouse/Rabbit Antibody on Rodent Tissue, Geen/Red
In immunohistochemistry, the technique of double staining is widely employed to simultaneously detect and differentiate two distinct antigens within a single tissue sample. The 2-Color IF Kit is designed to use Emerald (Green) and Permanent Red (Red) dye effectively stain 2 different antigens on mouse tissue or cell samples when paired with user-supplied mouse and rabbit primary antibodies.
Information
Kit Contents
Mouse Primer (RTU)
HRP-Polymer anti-Mouse (RTU)
AP-Polymer anti-Rabbit (RTU)
Permanent Red Substrate (RTU)
Permanent Red Activator (5x)
Permanent Red Chromogen (100x)
Emerald Chromogen (RTU)
Mounting Medium (RTU)
HRP-Polymer anti-Mouse (RTU)
AP-Polymer anti-Rabbit (RTU)
Permanent Red Substrate (RTU)
Permanent Red Activator (5x)
Permanent Red Chromogen (100x)
Emerald Chromogen (RTU)
Mounting Medium (RTU)
Usage
Inside the kit, you will find two polymer enzyme conjugates: Mouse AP Polymer and Rabbit HRP Polymer. Each conjugate is designed to work with specific substrates/chromogens. The Mouse AP Polymer is compatible with BCIP/NBT, which gives a purple color, while the Rabbit HRP Polymer is compatible with AEC, resulting in a green color.
Applications
Paraffin tissue (verified), frozen specimen and freshly prepared monolayer cell smears.
Color
Emerald (green) and Permanentred (red)
Specificity
Mouse and Rabbit
Tissue Species
Rodent
Storage
2-8℃
Note
The outcome is significantly influenced by several factors, including fixation, tissue slide thickness, antigen retrieval, as well as the dilution and incubation time of the primary antibody. It is crucial for the investigator to carefully evaluate all of these variables and establish the ideal conditions in order to accurately interpret the results.
Principle
Both enzyme conjugates are simultaneously applied to the specimen and mixed on the slide. This kit provides a streamlined protocol that is quicker and simpler than a sequential procedure. It utilizes a non-biotin system to prevent non-specific binding of endogenous biotin.