2-Color IHC Kit for Mouse/Rat Antibody on Human Tissue, Geen/Red
In immunohistochemistry, the technique of double staining is widely employed to simultaneously detect and differentiate two distinct antigens within a single tissue sample. The 2-Color IF Kit is designed to use Emerald (Green) and Permanent Red (Red) dye effectively stain 2 different antigens on human tissue or cell samples when paired with user-supplied mouse and rat primary antibodies.
Information
Kit Contents
Rat Primer (RTU)
AP-Polymer anti-Rat (RTU)
HRP-Polymer anti-Mouse (RTU)
DAB Substrate (RTU)
DAB Chromogen (20x)
Permanent Red Substrate (RTU)
Permanent Red Activator (5x)
Permanent Red Chromogen (100x)
Mounting Medium (RTU)
AP-Polymer anti-Rat (RTU)
HRP-Polymer anti-Mouse (RTU)
DAB Substrate (RTU)
DAB Chromogen (20x)
Permanent Red Substrate (RTU)
Permanent Red Activator (5x)
Permanent Red Chromogen (100x)
Mounting Medium (RTU)
Usage
The kit provides the user with two polymer enzyme conjugates: an anti-Mouse IgG (with minimal cross-reactivity to rat) HRP polymer and an anti-rat IgG (with minimal cross-reactivity to mouse) AP polymer. These conjugates come with two different substrates/chromogens: Permanent Red and DAB. The Permanent Red substrate reacts with the anti-Rat AP polymer conjugate, resulting in a red color, while the DAB chromogen reacts with the anti-Mouse HRP polymer conjugate, producing a brown color. To enhance the specificity of antibody staining, a Primer step is included. This kit utilizes a non-biotin system, eliminating the need for additional steps to block non-specific binding caused by endogenous biotin.
Applications
Paraffin tissue (verified), frozen specimen and freshly prepared monolayer cell smears.
Color
Emerald (green) and Permanentred (red)
Specificity
Mouse and Rat
Tissue Species
Human
Storage
2-8℃
Note
The outcome is significantly influenced by several factors, including fixation, tissue slide thickness, antigen retrieval, as well as the dilution and incubation time of the primary antibody. It is crucial for the investigator to carefully evaluate all of these variables and establish the ideal conditions in order to accurately interpret the results.