2-Color IHC Kit for Mouse/Rat Antibody on Rodent Tissue, Brown/Red
In immunohistochemistry, the technique of double staining is widely employed to simultaneously detect and differentiate two distinct antigens within a single tissue sample. The 2-Color IF Kit is designed to use DAB (brown) and Permanent Red (red) dye effectively stain 2 different antigens on mouse tissue or cell samples when paired with user-supplied mouse and rat primary antibodies.
Information
Kit Contents
HRP-Polymer anti-Mouse (RTU)
AP-Polymer anti-Rat (RTU)
Permanent Red Substrate (RTU）
Permanent Red Activator (5x)
Permanent Red Chromogen (100x)
Mouse-Rat Blocker 1 (RTU)
Mouse-Rat Blocker 2 (RTU)
Mouse Primer (RTU)
DAB Substrate (RTU)
DAB Chromogen (20x)
Mounting Medium (RTU)
AP-Polymer anti-Rat (RTU)
Permanent Red Substrate (RTU）
Permanent Red Activator (5x)
Permanent Red Chromogen (100x)
Mouse-Rat Blocker 1 (RTU)
Mouse-Rat Blocker 2 (RTU)
Mouse Primer (RTU)
DAB Substrate (RTU)
DAB Chromogen (20x)
Mounting Medium (RTU)
Usage
Included in the kit are two polymer enzyme conjugates: Mouse HRP Polymer and Rat AP Polymer. Each conjugate is accompanied by a specific substrate/chromogen. The Mouse HRP Polymer utilizes DAB, which creates a brown color, while the Rat AP Polymer uses Permanent Red, resulting in a red color.
Applications
Paraffin tissue (verified), frozen specimen and freshly prepared monolayer cell smears.
Color
DAB (brown) and Permanentred (red)
Specificity
Mouse and Rat
Tissue Species
Mouse
Storage
2-8℃
Note
The outcome is significantly influenced by several factors, including fixation, tissue slide thickness, antigen retrieval, as well as the dilution and incubation time of the primary antibody. It is crucial for the investigator to carefully evaluate all of these variables and establish the ideal conditions in order to accurately interpret the results.
Principle
A primer step is used to increase the specificity of antibody staining. This kit provides a simplified protocol that is quicker and easier than a sequential procedure. It utilizes a non-biotin system to prevent non-specific binding of endogenous biotin.