2-Color IHC Kit for Mouse/Rat Antibody on Rodent Tissue, Geen/Red
In immunohistochemistry, the technique of double staining is widely employed to simultaneously detect and differentiate two distinct antigens within a single tissue sample. The 2-Color IF Kit is designed to use Emerald (Green) and Permanent Red (Red) dye effectively stain 2 different antigens on mouse tissue or cell samples when paired with user-supplied mouse and rat primary antibodies.
Information
Kit Contents
HRP-Polymer anti-Mouse (RTU)
AP-Polymer anti-Rat (RTU)
Permanent Red Substrate (RTU)
Permanent Red Activator (5x)
Permanent Red Chromogen (100x)
Mouse-Rat Blocker 1 (RTU)
Mouse-Rat Blocker 2 (RTU)
Mouse Primer (RTU)
Emerald Chromogen (RTU)
Mounting Medium (RTU)
AP-Polymer anti-Rat (RTU)
Permanent Red Substrate (RTU)
Permanent Red Activator (5x)
Permanent Red Chromogen (100x)
Mouse-Rat Blocker 1 (RTU)
Mouse-Rat Blocker 2 (RTU)
Mouse Primer (RTU)
Emerald Chromogen (RTU)
Mounting Medium (RTU)
Usage
The kit supplies two polymer enzyme conjugates: Mouse HRP Polymer and Rat AP Polymer. Each conjugate is designed to work with specific substrates/chromogens. The Mouse HRP Polymer uses Emerald, resulting in a green color, while the Rat AP Polymer uses Permanent Red, producing a red color.
Applications
Paraffin tissue (verified), frozen specimen and freshly prepared monolayer cell smears.
Color
Emerald (green) and Permanentred (red)
Specificity
Mouse and Rat
Tissue Species
Mouse
Storage
2-8℃
Note
The outcome is significantly influenced by several factors, including fixation, tissue slide thickness, antigen retrieval, as well as the dilution and incubation time of the primary antibody. It is crucial for the investigator to carefully evaluate all of these variables and establish the ideal conditions in order to accurately interpret the results.
Principle
A primer step is employed to increase the specificity of antibody staining. This kit utilizes a non-biotin system to prevent non-specific binding of endogenous biotin.