2-Color IHC Kit for Rabbit/Rat Antibody on Human/Mouse Tissue, Geen/Red
In immunohistochemistry, the technique of double staining is widely employed to simultaneously detect and differentiate two distinct antigens within a single tissue sample. The 2-Color IF Kit is designed to use Emerald (Green) and Permanent Red (Red) dye effectively stain 2 different antigens on human and mouse tissue or cell samples when paired with user-supplied rabbit and rat primary antibodies.
Information
Kit Contents
HRP-Polymer anti-Rat (RTU）
AP-Polymer anti-Rabbit (RTU）
Permanent Red Substrate (RTU)
Permanent Red Activator (5x)
Permanent Red Chromogen (100x)
Emerald Chromogen (RTU)
Mounting Medium (RTU)
AP-Polymer anti-Rabbit (RTU）
Permanent Red Substrate (RTU)
Permanent Red Activator (5x)
Permanent Red Chromogen (100x)
Emerald Chromogen (RTU)
Mounting Medium (RTU)
Usage
The kit offers the user two polymer enzyme conjugates: HRP polymer anti-Rat IgG (minimal cross-reaction to mouse) and AP polymer anti-Rabbit IgG. These conjugates are accompanied by two distinct substrates/chromogens: Emerald and Permanent Red. Emerald chromogen reacts with the anti-Rat HRP polymer conjugate, producing a green color. Permanent Red reacts with anti-Rabbit AP polymer, producing a subsequent red color. When two proteins are co-localized, a blue/purple color will develop depending on which antigen is stronger. If only the rat antigen is present, only the Emerald chromogen will be present. Similarly, if the rabbit antigen is present, only the Permanent Red chromogenwill be present.
Applications
Paraffin tissue (verified), frozen specimen and freshly prepared monolayer cell smears.
Color
Emerald (green) and Permanentred (red)
Specificity
Rabbit and Rat
Tissue Species
Human and Mouse
Storage
2-8℃
Note
The outcome is significantly influenced by several factors, including fixation, tissue slide thickness, antigen retrieval, as well as the dilution and incubation time of the primary antibody. It is crucial for the investigator to carefully evaluate all of these variables and establish the ideal conditions in order to accurately interpret the results.