3-Color IHC Kit for 2 Mouse/1 Rabbit Antibody on Human Tissue, Brown/Red/Geen
Triple staining employs both conventional and innovative techniques in immunohistostaining to unveil the presence of three separate antigens and their simultaneous expression within a single tissue. The 3-Color IF Kit is designed to use DAB(brown), Permanent Red (red), and Emerald (green) dye effectively stain 3 different antigens on human tissue or cell samples when paired with user-supplied 2 mouse and 1 rabbit primary antibodies.
Information
Kit Contents
HRP-Polymer anti-Rabbit (RTU）
HRP-Polymer anti-Mouse (RTU）
AP-Polymer anti-Mouse (RTU）
DAB Substrate (RTU)
DAB Chromogen (20x)
Permanent Red Substrate (RTU)
Permanent Red Activator (5x)
Permanent Red Chromogen (100x)
Antibody Blocker (40x)
Emerald Chromogen (RTU)
Mouse-Mouse-Rabbit Blocker 1 (RTU)
Mouse-Mouse-Rabbit Blocker 2 (RTU)
Mounting Medium (RTU)
HRP-Polymer anti-Mouse (RTU）
AP-Polymer anti-Mouse (RTU）
DAB Substrate (RTU)
DAB Chromogen (20x)
Permanent Red Substrate (RTU)
Permanent Red Activator (5x)
Permanent Red Chromogen (100x)
Antibody Blocker (40x)
Emerald Chromogen (RTU)
Mouse-Mouse-Rabbit Blocker 1 (RTU)
Mouse-Mouse-Rabbit Blocker 2 (RTU)
Mounting Medium (RTU)
Usage
The kit provides polymer enzyme conjugates, including Polymer-HRP anti-rabbit, Polymer-AP anti-mouse, and Polymer-HRP anti-mouse, along with three chromogens: DAB (brown), Permanent Red (red), and Emerald (green). This kit utilizes a non-biotin system to prevent non-specific binding caused by endogenous biotin.
Applications
Paraffin tissue (verified), frozen specimen and freshly prepared monolayer cell smears.
Color
DAB (brown),Permanentred (red),and Emerald (green)
Specificity
Mouse and Rabbit
Tissue Species
Human
Storage
2-8℃
Note
The outcome is significantly influenced by several factors, including fixation, tissue slide thickness, antigen retrieval, as well as the dilution and incubation time of the primary antibody. It is crucial for the investigator to carefully evaluate all of these variables and establish the ideal conditions in order to accurately interpret the results.