3D Long-Term Live Imaging of Stem Cells
Stem cells are a diverse class of cells, including embryonic stem cells (ESC), tissue-specific stem cells (MSC) and induced pluripotent stem cells (iPS). Past studies have mainly used methods such as light microscopy, fluorescent probes and tissue sections to study the morphology and function of stem cells. However, these methods have limitations that prevent a full understanding of the diversity of stem cells, their involvement in disease modification, and their structural and cellular complexity in a given organism. Three-dimensional (3D) imaging has been shown to better maintain gene expression, cell polarity, and cell contact than traditional two-dimensional (2D) cell culture techniques.
Fig 1. 3D long-term live imaging of stem cell differentiation into neuroectoderm (Beghin, et al. 2021).
Services Overview
CD BioSciences offers optimized 3D long-term real-time imaging for stem cells, addressing the limitations of traditional fluorescence microscopy techniques that can lead to phototoxic stress and cellular damage. Our technology enables real-time non-invasive observation and documentation of the 3D structure and dynamic processes of live cells.
3D Long-Term Live Imaging of Stem Cells
Optimized phototoxicity for 3D long-time imaging
By minimizing the energy injected into the sample, we have reduced phototoxicity by a factor of 100. Helps our clients perform non-destructive real-time imaging and prolonged 3D imaging of undisturbed stem cells while maintaining spatial and temporal resolution.
Stem cell 3D image analysis
Utilize the inherent differences in cellular materials to measure the refractive index of different organelles and generate 3D images. Provides clients with important information about stem cell morphology, organelle distribution, intracellular activity, and interactions with the surrounding environment.
Stem cell dynamic detection
Assist clients in capturing the dynamic processes of stem cells, including cell division, migration, intracellular transport, etc. through continuous monitoring, helping clients to gain insight into the behavior and function of stem cells.
3D Long-Term Live Imaging of Stem Cells Workflow
Stem cell culture
Place the cells from the sample into the culture medium under appropriate conditions to promote cell proliferation .
Sample preparation
Place stem cells in appropriate 3D culture system for growth and differentiation.
Instrument setup
According to the experimental requirements and stem cell characteristics, perform imaging setup and adjust parameters such as time interval and duration.
Long-term imaging
Utilize the real-time imaging function to locate the stem cells of interest and start long-term live imaging to record the 3D structure and dynamic changes of the stem cells.
Data process and analyze
Automatically record and save image data in real time, process, analyze and visualize the data using relevant image process and analysis tools.
Deliverables
- Raw images of stem cells
- Quantitative analysis report
Our Advantages
- Costly labeling steps are not required, avoiding time-consuming and complexity and making the experimental process more efficient.
- Non-invasive observation of stem cells, which can be maintained in their natural state and can be imaged in 3D space.
- No time limitations, allowing long-term monitoring and understanding of the dynamic processes of stem cell self-renewal and differentiation.
CD BioSciences has a professional team and advanced imaging equipment to provide customers with accurate and reliable 3D long-term live imaging of stem cells. Help researchers to deeply understand the three-dimensional structure and dynamic process of stem cells, and promote the progress of scientific research. If you have any needs, please feel free to contact us.
- Beghin, et al. High content 3D imaging method for quantitative characterization of organoid development and phenotype. bioRxiv, 2021.
*If your organization requires the signing of a confidentiality agreement, please contact us by email.
Please note: Our services can only be used for research purposes. Do not use in diagnostic or therapeutic procedures!