3D Tissue Imaging
Conventional tissue analysis usually uses frozen or paraffin-embedded sample sections. Micron-thickness tissue sections allow individual cells to be labeled and characterized, but the three-dimensional characteristics of biological samples cannot be detected. The slice analysis only probes and studies the part of the pathological sample, and a considerable part of the information in the entire pathological tissue has not yet been explored. With the development of tissue transparency technology and the birth of light sheet microscopes, we can perform complete 3D imaging characterization of complete samples.
Figure 1. 3D image of spongy bone tissue affected by osteoporosis.
3D Tissue Imaging
Using traditional tissue analysis techniques to perform high-throughput sectioning and optical imaging analysis of tissue samples is expensive and difficult to obtain three-dimensional information. At CD BioSciences, we use confocal microscope or light sheet imaging to perform 3D imaging of tissue specimens processed by CLARITY technology. Our advanced experimental equipment and technology enable us to perform 3D imaging and phenotypic analysis of cell populations in the depth of the whole tissue, so as to provide better services for your biological research.
3D Tissue Imaging Workflow
In the process of 3D tissue imaging, we use CLARITY technology to clean the tissue without serial sectioning. This technology preserves spatial relationships and microstructures by forming a tissue-hydrogel mixture with increased permeability for deep optical interrogation, which can be used for three-dimensional (3D) mapping and the identification of rare markers. In addition, we also use professional image analysis software to analyze the results to get reliable results. The following is the workflow of 3D tissue imaging analysis.
3D immunolabeling
We have developed an optimized antibody labeling method so that we can achieve uniform labeling on thick tissues while maintaining histological integrity.
Step 1
Tissue removal
We use CLARITY tissue removal technology to make the organization transparent. The technique is easy to use and does not require continuous sectioning.
Step 2
3D imaging
Upon request, we can use immersion objectives, light sheet microscopes and other imaging methods to perform 3D imaging analysis on larger tissues.
Step 3
Quantitative image analysis
Although some of our customers only need raw data files from our imaging services, we also provide 3D image analysis services.
Step 4Delivery
Our Advantages
- Maintain the integrity of the tissue
- More accurate analysis of tissue samples
- Experienced scientists provide experimental consultation
- Reduced workload and cost
CD BioSciences has a professional team and advanced imaging equipment. We provide you with 3D tissue imaging services in various forms. You can send us fixed tissues for marking and imaging, or you can send us marked tissues for imaging. If you have any needs of 3D tissue imaging, please feel free to contact us.
- Chen Y, Shen Q, White S L, et al. Three-dimensional imaging and quantitative analysis in CLARITY processed breast cancer tissues[J]. Scientific reports, 2019, 9(1): 1-13.
- Zhang Y, Shin Y, Sung K, et al. 3D imaging of optically cleared tissue using a simplified CLARITY method and on-chip microscopy[J]. Science advances, 2017, 3(8): e1700553.
*If your organization requires the signing of a confidentiality agreement, please contact us by email.
Please note: Our services can only be used for research purposes. Do not use in diagnostic or therapeutic procedures!