4-Color mIF Staining Kit for Mouse Tissue, TSA
The tissue microenvironment contains a complex cellular composition. Different compositions can be detected and identified by multiplex immunofluorescence staining.
The 4-Color mIF Staining Kit is designed to utilize TSA technology to effectively detect 4 different antigens on mouse tissue or cell samples when paired with user-supplied rabbit primary antibodies.
Information
Kit Contents
TSA Green Dye (490nm/520nm)
TSA Red Dye (550nm/570nm)
TSA Orange Dye (640nm/660nm)or TSA Clear Dye (682nm/702nm)
Signal Amplification Reaction Buffer
Anti-Rabbit Antibody (HRP)
Mounting Medium
DAPI (368nm/461nm)
TSA Red Dye (550nm/570nm)
TSA Orange Dye (640nm/660nm)or TSA Clear Dye (682nm/702nm)
Signal Amplification Reaction Buffer
Anti-Rabbit Antibody (HRP)
Mounting Medium
DAPI (368nm/461nm)
Usage
The TSA dyes are 100×stock solution. Prepare a dye working solution by diluting the Signal Amplification Reaction Buffer at a 1:100 ratio (use as needed). Prepare a working solution of DAPI by diluting it with sterile water at a 1:100 ratio.
Applications
Can be used for immunofluorescence staining of tissues, paraffin sections, and TMA.
Color
Green/Red/OrangeDyeorClear/Blue
Specificity
Rabbit
Tissue Species
Mouse
Storage
Can be stored at 2-8℃ for 12 months.TSA Dye stored away from light.
Note
This reagent kit is for research use only. The activity of expired reagents may be reduced. Inadequate dewaxing can affect staining results. To prevent possible false negative or false positive results, positive and negative controls should be conducted simultaneously during the experiment.
Principle
This 4-Color mIF Staining Kit employs tyrosine signal amplification (TSA) technology, which utilizes HRP-labeled secondary antibodies to catalyze the fluorescein substrates to stably covalently bound and amplify the fluorescent signal to the antigen. The non-covalently bound antibodies are removed using the heat repair method, and a second round of incubation is performed with the next primary antibody and another fluorescent substrate, so that multiple labeling could be achieved. 