Serotonin is an important neurotransmitter, which is involved in regulating a wide range of physiological functions. The disorder of serotonin may cause a variety of psychiatric diseases, such as depression, schizophrenia, bipolar disorder, migraine and so on. 5-hydroxytryptamine receptor is a protein located on the cell membrane. Endogenous ligands, drug molecules and lipids are very important for the regulation and maintenance of its function. The physiological function of serotonin is mediated by more than a dozen 5-hydroxytryptamine receptors. Therefore, imaging analysis of the molecular structure and functional mechanism of 5-hydroxytryptamine receptors will bring new hope for the treatment of psychiatric diseases.

Figure 1. Cryo-electron microscopy structures of 5-hydroxytryptamine receptors bound to different ligands (Xu P, et al. 2021).

5-Hydroxytryptamine Receptors Imaging Analysis

In the past, scientists had little understanding of the fine structure of the serotonin receptor, making the development of new targeted drugs difficult. Co-immunoprecipitation, resonance energy transfer (RET) and bimolecular fluorescence complementation (BiFC) are commonly used methods to study the structure of 5-hydroxytryptamine receptor dimers, tetramers and higher-order oligomers. Co-immunoprecipitation requires solubilized proteins and cannot be applied to live cells, RET and BiFC can be applied to live cells, but they cannot directly measure protein-protein interactions. In order to develop more effective and less toxic therapeutic drugs, single-particle cryo-electron microscopy and fluorescence correlation spectroscopy are used to study the structure of 5-hydroxytryptamine receptors.

CD BioSciences has been committed to the development of fluorescent dyes and imaging technology for many years. We can study the structures of 5-hydroxytryptamine receptor-ligand complexes and 5-hydroxytryptamine receptor dimers by single particle cryo-electron microscopy, confocal microscopy and fluorescence correlation spectroscopy techniques, providing a theoretical basis for drug development targeting the 5-hydroxytryptamine receptor.

5-Hydroxytryptamine Receptors Imaging Analysis Workflow

The following are the specific steps of 5-hydroxytryptamine receptors imaging analysis

Delivery

High quality images of 5-hydroxytryptamine receptors
5-hydroxytryptamine receptor structure and location
Diffusion coefficient and oligomer size of 5-hydroxytryptamine receptors
Assessment of 5-hydroxytryptamine receptor oligomerization
Other data that you need

Our Advantages

• Live-cell assays with single-molecule sensitivity
• High-resolution, high-quality images
• Experienced scientists provide experimental consultation
• Reasonable price and short turnaround time

CD BioSciences has a professional team and advanced imaging equipment. The entire process of 5-hydroxytryptamine receptors imaging analysis is operated by experienced technicians to ensure the accuracy of the experiment. If you have any needs, please feel free to contact us.

1. Xu P, Huang S, Zhang H, et al. Structural insights into the lipid and ligand regulation of serotonin receptors[J]. Nature, 2021, 592(7854): 469-473.
2. Herrick-Davis K, Grinde E, Lindsley T, et al. Oligomer size of the serotonin 5-hydroxytryptamine 2C (5-HT2C) receptor revealed by fluorescence correlation spectroscopy with photon counting histogram analysis: evidence for homodimers without monomers or tetramers[J]. Journal of Biological Chemistry, 2012, 287(28): 23604-23614.

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Please note: Our services can only be used for research purposes. Do not use in diagnostic or therapeutic procedures!