ATP Metabolism Imaging Services
Cancer cells alter metabolic signaling pathways to obtain the energy needed for continued proliferation, and these metabolic signaling pathways are potential drug targets. ATP serves as a direct energy source for cellular metabolism, and developing therapies based on abnormal ATP metabolism has become an active research area for cancer and other diseases. However, current technology limits the assessment of changes in energy metabolism and interactions with the environment during proliferation in complex cancer cell models. ATP metabolism imaging technology can address these limitations and help us study intracellular energy production and dynamics process.
ATP Metabolism Imaging Services
CD BioSciences provides clients with the live cell imaging and analysis technology that directly analyzes ATP metabolism, allowing clients to gain insight into metabolic changes in cancer cells. Our services include the following
- Using live-cell imaging and analysis techniques to directly measure intracellular ATP levels and considering the effects of physiological environmental conditions on cellular metabolism that may introduce human factors, providing accurate and reliable metabolic information.
- Qualitative analysis in combination with cell morphology distinguishes the characteristics of ATP metabolic changes in different cell types, helping our clients to better understand the metabolic status in different cell types.
- Through continuous imaging and analysis, we are able to kinetically assess ATP metabolic changes in specific cell types in complex co-culture models, obtaining temporal changes in cellular metabolism.
- Direct measurement of intracellular cytoplasmic ATP to characterize the metabolic state of tumor cell models under physiologically relevant conditions.
ATP Metabolism Imaging Workflow
Experimental design
Determine cell type, treatment conditions, and analysis time points.
Cell culture and treatment
According to experimental requirements, perform cell culture and treat cells with specific conditions.
Acquire images
Capture images over time in physiologically relevant environments.
ATP metabolism analysis
Perform long-term/short-term real-time dynamic monitoring and generate ATP metabolism data and concentration response curves.
Confirm cell morphology
Qualitative examination of cell morphology using high-definition phase images, linking changes in cell morphology to ATP metabolism, revealing informative, temporal changes in cell behavior.
Deliverables
- Cell morphology images
- ATP metabolism data
- IC50 curve
Our Advantages
- Use non-interfering reagents to achieve dynamic analysis of ATP in living cells.
- Capture and analyze cell type-specific ATP changes to differentiate the effects of different cell types in advanced cell models.
- Measurements of ATP are generated in real time to conduct time studies, gain a comprehensive understanding of the impact of treatment or other factors on intracellular ATP levels.
- Allows the use of high-definition phase images at each time point to examine changes in cell morphology, obtain dynamic ATP measurement data, and at the same time, observe and record changes in cell morphology.
CD BioSciences has a professional team and advanced imaging equipment. The entire process of ATP metabolism imaging analysis is operated by experienced technicians to ensure the accuracy of the experiment. If you have any needs, please feel free to contact us, we can provide you with high-quality ATP metabolism imaging analysis services.
*If your organization requires the signing of a confidentiality agreement, please contact us by email.
Please note: Our services can only be used for research purposes. Do not use in diagnostic or therapeutic procedures!