Bacterial cells are highly organized. They can precisely control their cell organization and place them in appropriate locations inside and outside the cells to perform their normal functions. In rod-shaped bacteria, the cell is the core organization of cell regulation, which plays an important role in the cell cycle, cell differentiation, active transport, chemotaxis, and appendage growth. The polarized proteins in bacteria interact with other proteins or protein complexes already located there to form cell poles at the two poles of the bacteria. Therefore, imaging analysis of polar proteins in bacterial cells is essential for understanding the mechanism of bacterial polarity control.

Figure 1. Localization of polar proteins through the recognition of polar features (Altinoglu I, et al. 2019).

Bacterial Cell Pole Proteins Imaging Analysis

Fluorescence microscopy is a powerful tool for live cell imaging, which allows the detection of protein interactions and dynamics at the single cell level and bacterial populations. However, optical microscopy is limited by the diffraction limit and cannot analyze the interaction between molecules at the single-molecule level. In recent years, super-resolution microscopy has become an effective tool for cell structure and single-molecule dynamics analysis. It can accurately present the cell outline, inner membrane or periplasm structure, and study the mechanism of molecular interaction.

CD BioSciences has been committed to the development of fluorescent dyes and imaging technology for many years. We label the bacterial inner membrane or periplasm with a switchable fluorescent protein, and then use super-resolution imaging technology to describe the outline of the bacterial cell and the location of the polar protein, and then study the formation process and mechanism of the bacterial cell pole.

Bacterial Cell Pole Proteins Imaging Analysis Workflow

The following are the specific steps of bacteria cell pole proteins imaging analysis:

Delivery

Images obtained by super-resolution imaging of bacteria
Polar protein expression and fine mapping analysis
Two-dimensional map and histogram of the positioning of polar proteins
Inner curvature of bacterial cells
Percentage of polar molecules in a specific location
Other data that you need

Our Advantages

• High-throughput imaging and analysis
• Draw accurate contours of bacterial cells
• Track the trajectory of a single molecule
• Experienced scientists provide experimental consultation
• Reasonable price and short turnaround time

CD BioSciences has a professional team and advanced imaging equipment. The entire process of bacteria cell pole proteins imaging analysis is operated by experienced technicians to ensure the accuracy of the experiment. If you have any needs, please feel free to contact us.  

1. Altinoglu I, Merrifield C J, Yamaichi Y. Single molecule super-resolution imaging of bacterial cell pole proteins with high-throughput quantitative analysis pipeline[J]. Scientific reports, 2019, 9(1): 1-11.
2. Laloux G, Jacobs-Wagner C. How do bacteria localize proteins to the cell pole? [J]. Journal of cell science, 2014, 127(1): 11-19.

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Please note: Our services can only be used for research purposes. Do not use in diagnostic or therapeutic procedures!