Bacterial membrane vesicles (BMV) are spherical membrane structures that are ubiquitous in all bacterial species, including outer membrane vesicles and membrane vesicles. They are produced by Gram bacteria through different mechanisms. Outer membrane vesicles are vesicles derived from the outer membrane of gram-negative bacteria, and membrane vesicles are vesicles released from the plasma membrane of gram-positive bacteria. BMV can deliver molecules such as proteins, RNA and DNA to target prokaryotic or eukaryotic cells to mediate communication between bacteria or host-pathogen interactions. Therefore, imaging and tracking of bacterial membrane vesicles is very important for the treatment of bacterial-related diseases.

Figure 1. Visualization and tracking of MVs' behavior in live bacteria (Bos J, et al. 2021).

Bacterial Membrane Vesicles Imaging Analysis

For a long time, due to the lack of powerful imaging and tracking tools, the mechanism of bacterial membrane vesicles perform their functions and convey information is still unclear. However, with the continuous development of microscopes, it has become a powerful tool for live cell imaging and analysis, and researchers have a new understanding of the biological mechanisms related to vesicles.

CD BioSciences has been committed to the development of fluorescent dyes and imaging technology for many years. In order to study the dynamics of bacterial membrane vesicles, we quickly collected the images of fluorescent labeled bacterial membrane vesicles by high-power delayed fluorescence microscope, and imaged their motion trajectories by particle tracking technology.

Bacterial Membrane Vesicles Imaging Analysis Workflow

The following are the specific steps of bacteria membrane vesicles imaging analysis:

Delivery

Fluorescence image of bacterial membrane vesicles
Tracking analysis of bacterial membrane vesicles of different movement types
The trajectory of bacterial membrane vesicles
The positioning of bacterial membrane vesicles relative to the cell membrane
The influence of different conditions on the movement of bacterial membrane vesicles
Bacterial death, membrane depolarization, and membrane fluidity quantification assays
Other data that you need

Our Advantages

• High resolution, high signal-to-noise ratio
• Real-time imaging and tracking of bacterial membrane vesicles
• Experienced scientists provide experimental consultation
• Reasonable price and short turnaround time

CD BioSciences has a professional team and advanced imaging equipment. The entire process of bacteria membrane vesicles imaging analysis is operated by experienced technicians to ensure the accuracy of the experiment. If you have any needs, please feel free to contact us.  

1. Bos J, Cisneros L H, Mazel D. Real-time tracking of bacterial membrane vesicles reveals enhanced membrane traffic upon antibiotic exposure[J]. Science advances, 2021, 7(4): eabd1033.
2. Bitto N J, Zavan L, Johnston E L, et al. Considerations for the Analysis of Bacterial Membrane Vesicles: Methods of Vesicle Production and Quantification Can Influence Biological and Experimental Outcomes[J]. Microbiology Spectrum, 2021, 9(3): e01273-21.

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Please note: Our services can only be used for research purposes. Do not use in diagnostic or therapeutic procedures!