Cardiotoxicity Imaging Analysis
Cardiotoxicity characterized by severe cardiac dysfunction is a major problem for patients treated with different types of anticancer drugs. The current in vivo animal models and cell lines are not always sufficient to represent human biology. Therefore, it is important to develop methods and screening models for analyzing drug-induced cardiotoxicity. Human-induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CM) have shown great potential in drug-induced cardiotoxicity analysis.
Figure 1. 3D model of the heart.
Cardiotoxicity Imaging Analysis
Traditional fluorescence-based high-throughput cardiotoxicity detection technology can analyze the average signal from many cells regardless of the cell heterogeneity in hiPSC-CM. In order to grasp the dynamics of the cell population, CD BioSciences can quantitatively analyze the cardiotoxicity of hiPSC-CM through a fully automated imaging analysis system. This high-throughput system performs single-cell segmentation through nuclear signal extraction, and provides a solution that focuses on single-cell analysis by providing phenotypic output (such as single cell number/morphological characteristics).
| Analysis method | Fully automated imaging analysis |
| Analysis instrument | Fully automated imaging analysis system |
| Materials | HiPSC-CM, 96 well special optics plate, DMSO, paraformaldehyde, DPBS, other materials |
| Toxicity markers | Cell viability, cell apoptosis, cell morphology changes, others |
Cardiotoxicity Imaging Analysis Workflow
Most of the high-throughput image analysis used in hiPSC-CM research involves manual steps, mainly setting thresholds for positive signals or using commercial image analysis systems to segment individual cell nuclei. CD BioSciences uses fully automated high-throughput image analysis technology to analyze cytotoxicity. The detailed steps are as follows:
Step 1
Cell culture
The hiPSC-CM obtained on the 14th day of differentiation was thawed and seeded in a 96-well special optical plate in a humidified incubator at 37 ℃ and 5% CO2 for 24 hours.
Step 2
Add cardiac cytotoxicity inducer
HiPSC-CM used dimethyl sulfoxide as a control, treated with different concentrations of drugs for 4 days, and fixed in 2% paraformaldehyde for 30 minutes at room temperature.
Step 3
Imaging
Use high-content imaging equipment to obtain images of cardiomyocytes under different conditions.
Step 4
Image analysis
ImageJ software was used for image analysis, and the cardiotoxicity of the drug was evaluated through experimental data.
Delivery
Calcium signaling
Cell morphology data (area, circumference, elongation and density)
Our Advantages
- Fully automated high-throughput imaging can quickly acquire cell status
- Reduce the error caused by human operation
- Experienced scientists provide experimental consultation
- Reduced workload and cost
CD BioSciences has a professional team and advanced imaging equipment. The entire process of cardiotoxicity imaging analysis is operated by experienced technicians to ensure the accuracy of the experiment. If you have any needs, please feel free to contact us. We will design a personalized analysis plan for you according to your project, and provide you with accurate experimental data and analysis reports.
- Cao, Lu, et al. "Automated image analysis system for studying cardiotoxicity in human pluripotent stem cell-Derived cardiomyocytes." BMC bioinformatics 21 (2020): 1-12.
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Please note: Our services can only be used for research purposes. Do not use in diagnostic or therapeutic procedures!