Cell Migration Imaging Services
When a cell is affected by the interaction with neighboring cells, the movement of the cell is called collective cell migration. Cell migration plays a role in many key physiological processes, including gastrulation, embryo morphogenesis, nervous system development, tissue homeostasis, and immune cell transport. The movement or migration of cells is usually measured in vitro to clarify the mechanisms of various physiological activities.
Figure 1. Analysis of individual melanoma cells migration.
Cell Migration Imaging Analysis
Understanding collective cell migration is of key value for the treatment of many types of diseases. CD BioSciences can provide you with a series of imaging analysis solutions to characterize various forms of cell migration. Our method can provide robust and reproducible quantitative results of cell migration.
| Methods | Description | Workflow |
Scratch wound assay |
Wound healing or "scratch" assays are one of the most commonly used in vitro methods to monitor and quantify collective cell migration. We create uniform and precise wounds in 24-well plates or 96-well plates, and then use automatic microscopes or cell imaging multi-mode readers to monitor cell migration in the wound area over time. |
1. Capture: Kinetic high contrast brightfield (HCBF) images are captured based on desired interval and duration. |
Barrier cell migration assay |
The cell rejection zone assay or barrier assay relies on the creation of cell-free zones without the use of mechanical scratches. The removal of the temporary barrier allows surrounding cells to migrate to the open area. We use an automatic live cell imager or a cell imaging multi-mode reader to monitor the migration of cells to the wound area over time, and use imaging software to analyze the results of cell migration. |
1. Capture: Kinetic high contrast brightfield (HCBF) images are captured based on desired interval and duration. |
Chemotactic cell migration and invasion assay |
The emergence of osmotic supports provides a simple in vitro method to measure cell migration and invasion induced by chemoattractants. The addition of a fluorescence blocking membrane improves the accuracy of the measurement. We use an inverted fluorescence microscope for detailed kinetic analysis, which can accurately calculate the number of cells reaching the bottom of the membrane. |
1. Detection setup and analysis: Add the medium containing the chemical attractant to the fluorescently labeled cell culture. Dynamic fluorescence imaging and image analysis tools are used to characterize the migration of cells to the basolateral surface of the permeable carrier. |
Delivery
Wound area
Healing speed
Front cell velocity
Traveled Distance
Velocity
Invasion
Spreading rate
Other relevant data
Our Advantages
- Objective quantification of cell migration
- Generate high-throughput image data
- Experienced scientists provide experimental consultation
- Reasonable price and short turnaround time
CD BioSciences has a professional team and advanced imaging equipment. The entire process of cell migration imaging analysis is operated by experienced technicians to ensure the accuracy of the experiment. If you have any needs, please feel free to contact us.
- Pijuan, Jordi, et al. "In vitro cell migration, invasion, and adhesion assays: from cell imaging to data analysis." Frontiers in cell and developmental biology 7 (2019): 107.
*If your organization requires the signing of a confidentiality agreement, please contact us by email.
Please note: Our services can only be used for research purposes. Do not use in diagnostic or therapeutic procedures!