Cell Viability Imaging Services
Cell viability is a measure of the proportion of live and healthy cells in a cell population. Cell viability assays are used to determine the overall health of cells, optimize culture or experimental conditions, and measure the survival rate of cells after treatment with compounds, for example during drug screening. Generally, cell viability assays provide a reading of cell health by measuring metabolic activity, ATP content, or cell proliferation. Cytotoxicity assays can also be used to assess cell viability, which provides readings of cell death markers, such as loss of membrane integrity.
Figure 1. Cell viability assays by MTT.
Cell Viability Assays by Imaging Technology
CD BioSciences provides a variety of cell viability assays methods based on imaging technology to determine cell viability in monolayer cell cultures, 3D microtissues, primary cells, stem cells, and bacterial cultures. We can provide you with high-quality cell viability assays services with accurate and repeatable results to determine the health of your cells.
Cell Viability Assays Methods
| Flow cytometry | High-content imaging | Trypan blue | |
|---|---|---|---|
| Details | Dead cells can nonspecifically bind to many reagents, resulting in false positive results. Therefore, removing dead cells from flow cytometry data is a key step to ensure the accuracy of results and analysis, so as to more accurately distinguish between living cells and dead cell populations. | High-content imaging can provide superior single-cell analysis capabilities and lightning-fast data acquisition time. It can perform inactive analysis of intact, fixed or live cells from monolayers to spheroids. | The principle of the trypan blue method is that living cells do not absorb blue dye and dead cells will absorb blue dye. Cell viability can be calculated by the ratio of total viable cells/total cells. Staining also facilitates the visualization of the overall cell morphology. |
| Application | Bacterial cells Yeast cells |
Breast cancer tissue cells HeLa cells A549 cells BPAE cells |
Most mammalian cells |
Cell Viability Assays Workflow
We can use a variety of imaging techniques to assay cell viability. The general workflow is as follows:
Step 1
Experiment plan
Design your experiment, considering the instruments and reagents required for each step.
Step 2
Cell culture
Let target cells grow under the most suitable conditions.
Step 3
Cell marker
Use selective specific fluorescent dyes to label target cell structure.
Step 4
Acquire cell images
Capture target cell images with maximum clarity and resolution.
Step 5
Cell viability analysis
Use the imaging analysis software to analyze cell viability.
Our Advantages
- Provide different types of cell viability assay methods, designed to suit your experimental design
- A variety of colors are available, compatible with most instrument configurations
- Quickly obtain complex images and high-content data
- The experimental results are accurate and repeatable
- Reasonable price
CD BioSciences has a professional team and advanced imaging equipment, and the entire process of cell viability assay is operated by experienced technicians in order to ensure the accuracy of the experiment. If you have any needs, please feel free to contact us.
*If your organization requires the signing of a confidentiality agreement, please contact us by email.
Please note: Our services can only be used for research purposes. Do not use in diagnostic or therapeutic procedures!