Chemokine receptors are members of the G protein-coupled receptor superfamily with seven transmembrane domains. Chemokines are a large class of small proteins that regulate leukocyte function by binding to specific chemokine receptors. There are nearly 50 chemokines and more than 20 chemokine receptors in the human body, and one chemokine can bind to many different chemokine receptors, and vice versa. Their interactions constitute complex regulatory networks that mediate cell migration and are closely related to diseases such as inflammation and cancer. Therefore, imaging analysis of chemokine receptors is important for drug development and disease treatment.

Figure 1. Overall structure comparison between inactive and active CXCR2 (Liu K, et al. 2021).

Chemokine Receptors Imaging Analysis

In recent years, researchers have used advanced fluorescence microscopy to study the oligomerization of chemokine receptors, ranging from homodimers and homooligomers to heterodimers. Studies have shown that the dimerization of many chemokine receptors has dynamic properties.

CD BioSciences has been committed to the development of fluorescent dyes and imaging technology for many years. We can study the expression and structure of chemokine receptor dimer on the surface of living cells by FRET imaging, quantitative microanalysis, single molecule microscope and fluorescence fluctuation spectroscopy. In addition, we also studied the stability, kinetics and function of chemokine receptor dimer. This will help to develop new strategies to design chemokine receptor targeted drugs.

Chemokine Receptors Imaging Analysis Workflow

The following are the specific steps of chemokine receptors imaging analysis

Delivery

High-quality images of chemokine receptor monomers, dimers, and oligomers
Ratio of monomers, dimers and oligomers of chemokine receptors
Factors influencing chemokine receptor dimerization
Molecular dynamics of chemokine receptor dimerization
Other data that you need

Our Advantages

• Ability to visualize chemokine receptors with single-molecule precision
• Provides high-quality images and precise analysis results
• Experienced scientists provide experimental consultation
• Reasonable price and short turnaround time

CD BioSciences has a professional team and advanced imaging equipment. The entire process of chemokine receptors imaging analysis is operated by experienced technicians to ensure the accuracy of the experiment. If you have any needs, please feel free to contact us.  

1. Liu K, Wu L, Yuan S, et al. Structural basis of CXC chemokine receptor 2 activation and signaling[J]. Nature.
2. Nilgun I, Dale H, Tian J, et al. Fluorescence resonance energy transfer imaging reveals that chemokine-binding modulates heterodimers of CXCR4 and CCR5 receptors[J]. Plos One, 2008, 3(10): e3424.
3. Isbilir A, Mller J, Arimont M, et al. Advanced fluorescence microscopy reveals disruption of dynamic CXCR4 dimerization by subpocket-specific inverse agonists[J]. Proceedings of the National Academy of Sciences, 2020, 117(46).

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Please note: Our services can only be used for research purposes. Do not use in diagnostic or therapeutic procedures!