Cholestasis Imaging Analysis
Cholestasis is a pathophysiological process caused by the disorder of bile secretion and excretion. It is manifested in the excessive accumulation of bile components such as cholic acid, cholesterol and bilirubin in the liver and systemic circulation, resulting in damage to hepatocytes and the body. Long-term continuous cholestasis will progress to liver fibrosis and even liver cirrhosis. Imaging analysis is often used to check whether there is biliary obstruction and the specific cause of obstruction.
Figure 1. Morphological alterations in cholestasis. (Jansen, P.L., et al. 2017).
Cholestasis Imaging Analysis
Early lesions of the "downstream" bile duct lead to cholestasis and bile salt (BS) mediated "upstream" liver parenchymal toxic injury. Understanding the cellular physiology of bile secretion enables people to better grasp the cholestasis caused by various drugs. CD BioSciences can provide you with advanced cholestasis imaging analysis services. We use fluorescent bile acid probes and high-content imaging to directly measure BS accumulation, and then analyze the degree of bile duct expansion and contraction to evaluate the toxicity of candidate drugs.
| Analysis method | High content imaging |
| Cell types available | HepaRG, HepG2 Primary human hepatocytes, primary cynomolgus monkey hepatocytes, primary canine hepatocytes |
| Cell model | 2D cell monolayer |
| Sample requirements | 50µL of 20 mM solution or equivalent amount of solid |
Cholestasis Imaging Analysis Workflow
We use high-content imaging to analyze cholestasis caused by drug candidates, and the analysis steps are as follows:
Step 1
The cells were cultured to 80% confluent monolayer.
Step 2
Treatment with test compound.
Step 3
Addition of fluorescent bile acid probe.
Step 4
Cells were fixed and counterstained with DAPI.
Step 5
High content imaging and analyzing bile acid salt accumulation and bile duct size.
Delivery
Dose-response curves
EC50 values
Image depicting the accumulation of fluorescent bile salt probes in the bile duct
Our Advantages
- A variety of fluorescent probes can be used to evaluate the degree of bile salt accumulation
- Multiple types of data can be provided according to customer requirements
- High-throughput detection, simple and cost-effective
- Short turnaround time
- Experienced scientists provide experimental consultation
CD BioSciences has a professional team and advanced imaging equipment. The entire process of cholestasis imaging analysis is operated by experienced technicians to ensure the accuracy of the experiment. If you have any needs, please feel free to contact us, we can provide you with customized cholestasis imaging analysis services.
- Jansen PL, Ghallab A, Vartak N, et al. The ascending pathophysiology of cholestatic liver disease[J]. Hepatology, 2017, 65(2): 722-738.
*If your organization requires the signing of a confidentiality agreement, please contact us by email.
Please note: Our services can only be used for research purposes. Do not use in diagnostic or therapeutic procedures!