Chromosome Karyotype Imaging Services
Chromosomes are the hereditary material and important carriers of genes, with which the reproduction and development of organisms are closely related. Karyotype refers to the image formed by arranging all chromosomes of a somatic cell in the middle of mitosis according to their size and morphological features. Chromosome karyotype imaging analysis is an important research tool in cytogenetics. By observing variations in chromosome structure and number, it is possible to determine the cause of an organism's disease, for example, by identifying the missing genes that lead to a particular disease.
Fig. 1 Raw G-band chromosome image and its karyotype (Emrecan Altinsoy, et al. 2020).
Chromosome Karyotype Imaging Services
CD BioSciences provides customers with chromosomal karyotyping imaging analysis services, which is an important laboratory technique used to study the total number and structure of chromosomes in cells by observing them under a microscope. The sample types we can detect include:
- Human chromosomes (live cells, fixed cells, peripheral blood)
- Mouse chromosomes (fixed cells, peripheral blood, bone marrow, spleen)
Our services
Chromosome pairs, labels and groups
Utilizing advanced technology and imaging equipment, chromosomes are paired, labeled and grouped based on the inherent morphological characteristics of the target cells.
Chromosome morphology analysis
Chromosomes of different species have unique morphological features, such as chromosome number, length, centromere position, arm ratio and satellite size. These morphological features are relatively stable, and by staining or fluorescent labeling of chromosomes, we can observe the specific morphological structure of chromosomes clearly and intuitively using optical or electrochemical staining devices.
Chromosome abnormality detection
By comparing with the normal karyotype, we can detect chromosome abnormalities, such as deletion, duplication and inversion, and generate a detailed analysis report.
Chromosome Karyotype Analysis Method
Conventional morphological analysis
Taking metaphase chromosomes as the research object, the chromosomes are analyzed and compared based on their characteristics using banding technology.
Band analysis
The chromosome specimens fixed on the glass slide are treated with trypsin, stained with Giemsa, and the light and dark bands of the chromosomes are observed under a light microscope.
Segmental staining analysis
Chromosomes are processed by low temperature, KCl and enzymatic hydrolysis, HCl or a mixture of HCl and acetic acid, etc., and then sliced, so that the chromosomes undergo a heteropyknosis reaction and the heterochromatin segments are stained and visible.
Chromosome Karyotype Imaging Workflow
Sample delivery requirements
Sample collection
Receive the samples to be tested and collect and preserve the samples using appropriate methods.
Cell culture
Place the cells from the sample into the culture medium under appropriate conditions to promote cell proliferation.
Hypotonic treatment
Cells are briefly treated with a hypotonic solution to swell the nucleus and make it easier to identify each chromosome.
Cell fixation
Cells are fixed and dried using fixative solution.
Staining or labeling
The chromosomes in the cells are stained or labeled by staining techniques or specific fluorescent labeling probes. The most commonly used stain is Giemsa stain.
Imaging observation
Using high-resolution microscopes and imaging equipment, observe and take images of cellular chromosomes to obtain detailed morphological and structural information.
Chromosome karyotyping
Determine the number, structure, abnormalities and other information of chromosomes. Chromosome karyotype analysis methods mainly include conventional morphological analysis, banding analysis and coloring segment analysis.
- Live cells
Use a T25 culture flask to culture one bottle of live cells. The cell confluence is 50-60%. Fill it with culture medium. Mark the name and gender of the cells. Send it at room temperature, and send the culture medium at the same time.
- Fixed cells
Cell number >106, ice pack transportation.
- Peripheral blood
5mL of EDTA anticoagulant blood, transported with ice pack.
Deliverables
- Chromosome karyotype analysis images
- Karyotype analysis result report (including total number of chromosomes, structure and morphological characteristics)
- Chromosome abnormality detection report (including staining deletions, duplications, inversions, etc.)
CD BioSciences has a professional team and advanced imaging equipment to provide customers with accurate and reliable chromosome karyotype imaging analysis services. Help researchers gain an in-depth understanding of the chromosome structure and function of cells and promote the progress of scientific research. If you have any needs, please feel free to contact us.
- Emrecan Altinsoy, Jie Yang and Can Yilmaz, Fully-automatic raw G-band chromosome image segmentation. IET Image Processing, 2020, 14(9):1920-1928.
*If your organization requires the signing of a confidentiality agreement, please contact us by email.
Please note: Our services can only be used for research purposes. Do not use in diagnostic or therapeutic procedures!