The cytoskeleton consists of a complex intracellular scaffold composed of protein fibers called filaments. According to their specific structural and functional properties, three types of filaments can be distinguished: actin filaments, intermediate filaments and microtubules. The cytoskeleton is a highly dynamic and adjustable scaffold. In addition to serving as a cell scaffold, the cytoskeleton also plays a role in organelle transport, cell division, migration, and signal transduction. Abnormal cytoskeleton often leads to diseases. Therefore, imaging and analysis of cytoskeleton under different physiological and pathological conditions is very important.

Figure 1. Obtained cytoskeleton image can include some degrees of blurring (Alioscha-Perez M, et al. 2016).

Cytoskeleton Imaging Analysis

As one of the most basic and easy fluorescent labeling structures of cells, cytoskeleton is favored by researchers. Tubulin and actin, as important components of cytoskeleton, are the most frequently studied cytoskeleton proteins. Therefore, fluorescent labeled actin and microtubules are an effective means to visually analyze the dynamic structure of cytoskeleton.

In the past, the analysis of the cytoskeleton structure was generally performed by experts under a microscope to visually observe the stained cytoskeleton. However, these conventional methods are based on the subjective judgment of researchers and lack objectivity. In addition, as the number of specimens to be analyzed increases, the labor cost of experts also increases. In order to solve these problems, CD BioSciences uses microscope image analysis technology to automatically evaluate the quantitative method of complex cytoskeletal structure characteristics, so that it can accurately analyze the cytoskeleton structure.

Cytoskeleton Imaging Analysis Workflow

The following are the specific experimental steps of cytoskeleton imaging analysis:

Delivery

Cytoskeleton image
Normalized angular distribution of fibers grown in different stress conditions
Orientation of fibers and other relevant data

Our Advantages

• High-sensitivity optical system to capture high-quality pictures
• Fully automatic image analysis and data management
• Experienced scientists provide experimental consultation
• Reasonable price and short turnaround time

CD BioSciences has a professional team and advanced imaging equipment. The entire process of cytoskeleton imaging analysis is operated by experienced technicians to ensure the accuracy of the experiment. If you have any needs, please feel free to contact us. We will provide you with personalized cytoskeleton imaging analysis services according to your needs.

1. Alioscha-Perez M, Benadiba C, Goossens K, et al. A robust actin filaments image analysis framework[J]. PLoS computational biology, 2016, 12(8): e1005063.
2. Herberich G, Würflinger T, Sechi A, et al. Fluorescence microscopic imaging and image analysis of the cytoskeleton[C]//2010 Conference Record of the Forty Fourth Asilomar Conference on Signals, Systems and Computers. IEEE, 2010: 1359-1363.

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Please note: Our services can only be used for research purposes. Do not use in diagnostic or therapeutic procedures!