Deparaffinator 20X with Hot Rinse
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Catalog NO: IHA040106
Size: 100 mL; 1 L
Introduction:Deparaffinator 20X with Hot Rinse is part of a set of solutions intended for paraffin removal from paraffin-embedded tissues, hydrating and heat permeabilizing tissues to achieve epitope or nucleic acid retrieval. Deparaffinator 20X with Hot Rinse is an innovative reagent formulated to reduce exposure to toxic solvents and to reduce the number of steps in deparaffinization, hydration and epitope or nucleic acid retrieval, thus making them a safe and efficient alternative to traditional deparaffinization involving xylenes and alcohols. Since it do not interfere with the detection of proteins by Immunohistochemistry, Immunocytochemistry, or of nucleic acids by CISH and FISH, results are the same as when using xylene and alcohol. The deparaffinization procedure with Deparaffinator 20X with Hot Rinse uses a heat-assisted deparaffinization method while achieving tissue hydration and retrieval of proteins and nucleic acids. Deparaffinization at moderately elevated temperatures has been shown to adequately dissolve paraffin at significantly lower solvent concentrations compared to methods of deparaffinization at ambient temperatures using xylenes and/or xylene substitutes. By utilizing lower non-toxic solvent concentrations, the process of tissue rehydration following deparaffinization and retrieval can be achieved with a single final rinse step to remove micro paraffin leftovers, followed by direct transfer of slides to a buffer bath. These reagents are designed to deparaffinize, rehydrate and retrieve one microscope slide containing a standard size (from 0.5 cm x 1 cm up to 2.5 cm x 2.5 cm diameter) paraffin-embedded tissue section per 3 ml of Deparaffinator with Hot Rinse 1X. A typical staining dish containing 200-250 mL of the Deparaffinator with Hot Rinse 1X solution will help deparaffinize approximately 72 standard tissue sections. If larger tissue sections are used, the number of processed slides may be less. If residual paraffin is observed microscopically on the slides following deparaffinization, the deparaffinization reagents should be replaced.
Category:Deparaffinators, Retrievers and Enzymes
Presentation:Deparaffinator 20X with Hot Rinse contain glycols and glycol ethers. It is provided in liquid form.
Storage:Store in original tightly closed original container in a dry, cool and well-ventilated place. Containers which are opened must be carefully resealed and kept upright to prevent leakage. Refer to the product label. Store at 20-25 °C. DO NOT REFRIGERATE OR FREEZE.
Intended Use:For In Vitro Diagnostic Use Only.
Application:For paraffin removal from paraffin-embedded tissues, hydrating and heat permeabilizing tissues to achieve epitope or nucleic acid retrieval. Not intended for use in humans or animals.