Bacterial infection is a common clinical problem in acute and chronic wounds. Treatment of bacterial infections can only be done after an accurate diagnosis. Currently, the time-consuming bacterial culture may lead to unrecognized bacterial presence, delaying diagnosis. Therefore, it is very important to develop a rapid and accurate method for diagnosing bacterial infection. The combination of direct multiplex imaging of bacteria and Gram staining enables rapid and accurate diagnosis of bacterial infections.

Figure 1. Routine Gram stain shows identification of Gram negative (a P. aeruginosa) and Gram positive (b S. aureus) bacteria in both large aggregates (dotted lines) and individual bacteria within the biofilm (Becerra S C, et al. 2016).

Direct Multiplex Imaging Analysis of Bacterial Infections

Gram stain is a test that checks for bacteria at the site of infection. DMI can rapidly, specifically and reproducibly identify a variety of pathogenic microorganisms directly from samples, providing necessary information for timely and decisive treatment of diseases.

CD BioSciences has been committed to the development of fluorescent dyes and imaging technology for many years. We can combine Gram stain and DMI results to provide important information for the treatment of diseases caused by bacterial infections. Compared to other molecular diagnostic methods, our approach can reduce unnecessary concomitant treatment costs and save you time.

Detectable Bacterial Pathogens

Gram negative bacteria	Gram positive bacteria
Acinetobacter spp.	Staphylococcus spp.
Enterobacteriaceae	Staphylococcus aureus
Escherichia coli	Streptococcus spp.
Haemophilus influenzae	Streptococcus pneumoniae
Klebsiella pneumoniae	Enterococcus faecalis
Pseudomonas aeruginosa
Serratia marcescens
Stenotrophomonas maltophilia
Proteus mirabilis

The Workflow of Direct Multiplex Imaging Analysis of Bacterial Infections

The following are the specific steps of direct multiplex imaging analysis of bacterial infections

Delivery

High-quality sample images
Pathogen identification results
Other data that you need

Our Advantages

• Specificity and sensitivity
• Intact preserved cell morphology
• Allows phenotyping and viability assessment
• Experienced scientists provide experimental consultation
• Reasonable price and short turnaround time

CD BioSciences has a professional team and advanced imaging equipment. The entire process of bacterial infections imaging analysis is operated by experienced technicians to ensure the accuracy of the experiment. If you have any needs, please feel free to contact us.  

1. Becerra S C, Roy D C, Sanchez C J, et al. An optimized staining technique for the detection of Gram positive and Gram negative bacteria within tissue[J]. BMC Research Notes, 2016, 9(1).

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Please note: Our services can only be used for research purposes. Do not use in diagnostic or therapeutic procedures!