Transcription is the first step in gene expression. This process transcribes the genetic information in DNA into mRNA, and then mRNA is translated to produce specific proteins. The transcription process is influenced by a variety of regulatory factors and involves a delicate balance of multiple dynamic molecular interactions. Understanding the gene regulation and expression mechanism of DNA transcription is helpful to understand the pathogenesis of many diseases and develop corresponding treatment schemes. Therefore, it is very important to study DNA transcription on multiple scales by imaging technology.

Figure 1. Transcription is the process of copying a segment of DNA into RNA.

DNA Transcription Imaging Analysis

With the continuous development of single-molecule and single-cell technologies, many different technologies have been used to study DNA transcription in living cells. These technologies can visualize single RNA transcripts in real time and quantify the transcription process with single-molecule resolution. Single-molecule imaging in living cells has become an effective tool for DNA transcription imaging analysis, which can visualize the transcription process with higher molecular resolution.

CD BioSciences has been committed to the research and development of fluorescent probes and living cell imaging technology for many years. We can fluorescently label DNA molecules through chemical conjugation or intercalation of dyes, and then analyze the DNA transcription process by single molecule imaging with TIRF microscope.

DNA Transcription Imaging Analysis Workflow

The following are the specific steps of DNA transcription imaging analysis:

Delivery

Images of various stages of the transcription cycle
DNA secondary structure during transcription
Number of mRNA
TF-DNA interaction dynamics
Provide other data according to your needs

Our Advantages

• High spatial resolution, high signal-to-noise ratio
• Visually analyze molecular interactions
• Experienced scientists provide experimental consultation
• Reasonable price and short turnaround time

CD BioSciences has a professional team and advanced imaging equipment. The entire process of DNA transcription imaging analysis is operated by experienced technicians to ensure the accuracy of the experiment. If you have any needs, please feel free to contact us.

1. Lee C Y, Myong S. Probing steps in DNA transcription using single-molecule methods[J]. Journal of Biological Chemistry, 2021, 297(3).
2. Price R M, Budzyński M A, Kundra S, et al. Advances in visualizing transcription factor–DNA interactions[J]. Genome, 2021, 64(4): 449-466.

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Please note: Our services can only be used for research purposes. Do not use in diagnostic or therapeutic procedures!