The estrogen receptor (ER) is a ligand-activated transcription factor involved in the regulation of cell proliferation, differentiation and reproductive physiology. Breast cancer is one of the most common malignant tumors in women. 70% of breast cancers are estrogen-dependent tumors. Estrogen promotes breast cancer cell growth by interacting with estrogen receptors (ER, including both alpha and beta subtypes). In recent years, the estrogen receptor has been identified as the target of breast cancer therapeutic drugs, providing new methods and technical guidance for hormone replacement therapy of breast cancer and other diseases. However, the biological function of ER in disease progression remains unclear, so ER imaging analysis is critical for breast cancer treatment.

Figure 1. Estrogen receptor staining in breast cancer tissue microarrays by both chromogenic and fluorescent methods (Zarrella E R, et al. 2016).

Estrogen Receptors Imaging Analysis

At present, the interaction between the two subtypes of estrogen receptors ERα and ERβ, the biological roles of homodimers and heterodimers, and the mechanism of estrogen receptor-drug interactions are still need to be explored. CD BioSciences has been committed to the development of imaging technology and fluorescent probe for many years. We can study the dimerization of ERα and ERβ in cells based on the luciferase complementation system of the split reporter gene, and we can also study the function of ER in living cells and its related drug action mechanism through highly specific fluorescent probes. These studies will provide new ideas for the development of high-efficiency and low-toxicity estrogen receptor-targeting drugs.

Estrogen Receptors Imaging Analysis Workflow

The following are the specific steps of estrogen receptors imaging analysis

Delivery

High-quality images of estrogen receptor homodimers and heterodimers
Conditions for the formation of estrogen receptor homodimers and heterodimers
Kinetic analysis of estrogen receptors
Other data that you need

Our Advantages

• In situ visualization of estrogen receptors in living cells
• Development of highly specific fluorescent probes
• Experienced scientists provide experimental consultation
• Reasonable price and short turnaround time

CD BioSciences has a professional team and advanced imaging equipment. The entire process of estrogen receptors imaging analysis is operated by experienced technicians to ensure the accuracy of the experiment. If you have any needs, please feel free to contact us.  

1. Yang L, Meng Q, Hu Z, et al. Estrogen receptor sensing in living cells by a high affinity turn-on fluorescent probe[J]. Sensors and Actuators B: Chemical, 2018, 272: 589-597.
2. Paulmurugan R, Tamrazi A, Massoud T F, et al. In vitro and in vivo molecular imaging of estrogen receptor α and β homo-and heterodimerization: exploration of new modes of receptor regulation[J]. Molecular Endocrinology, 2011, 25(12): 2029-2040.
3. Zarrella E R, Coulter M, Welsh A W, et al. Automated measurement of estrogen receptor in breast cancer: a comparison of fluorescent and chromogenic methods of measurement[J]. Laboratory Investigation, 2016, 96(9): 1016-1025.

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Please note: Our services can only be used for research purposes. Do not use in diagnostic or therapeutic procedures!