GABA_A receptors are the main inhibitory neurotransmitter receptors in the central nervous system, which can inhibit nerve excitability by mediating the influx of extracellular chloride ions. The localization and distribution of GABA_A receptors on the nerve cell membrane plays an important role in regulating the generation and transmission of nerve signals. A large number of studies have shown that the dysregulation of GABA_A receptors in the cell membrane distribution level is closely related to the occurrence of neurological diseases. In addition, GABA_A receptors are also important drug targets for central nervous system diseases. Therefore, imaging analysis of the structure and transport of GABA_A receptors is of great importance.

Figure 1. Overall structure of the GABA_A receptor-Fab complex (Zhu S, et al. 2018).

GABA_A Receptors Imaging Analysis

For a long time, X-ray diffraction has been regarded as the gold standard of structural biology. It requires protein crystallization and determines the protein structure according to the X-ray diffraction pattern. However, the three-dimensional structure of GABA_A receptor is difficult to be analyzed by X-ray diffraction crystal analysis.

CD BioSciences has been committed to the development of imaging technology and fluorescent dye for many years. We can use confocal fluorescence microscopy and high-resolution electron microscopy to characterize the three-dimensional structure of GABA_A receptors in cells. In addition, we can track the movement of individual GABA_A receptors within living cells via genetically encoded fluorophores.

GABA_A Receptors Imaging Analysis Workflow

The following are the specific steps of GABA_A receptors imaging analysis

Delivery

High-resolution microscope images of GABA_A receptors
High-resolution structure of the GABA_A receptors
Location and transport of intracellular GABA_A receptors
Other data that you need

Our Advantages

• Studying the spatial distribution of cell surface GABA_A receptors at the nanoscale
• Live cell imaging can visually analyze receptor trafficking
• Experienced scientists provide experimental consultation
• Reasonable price and short turnaround time

CD BioSciences has a professional team and advanced imaging equipment. The entire process of GABA_A receptors imaging analysis is operated by experienced technicians to ensure the accuracy of the experiment. If you have any needs, please feel free to contact us.  

1. Orlando M, Ravasenga T, Petrini E M, et al. Correlating fluorescence and high-resolution scanning electron microscopy (HRSEM) for the study of GABA_A receptor clustering induced by inhibitory synaptic plasticity[J]. Scientific Reports, 2017, 7(1): 1-10.
2. Zhu S, Noviello C M, Teng J, et al. Structure of a human synaptic GABA_A receptor[J]. Nature, 2018, 559(7712): 67-72.
3. Lorenz-Guertin J M, Wilcox M R, Zhang M, et al. A versatile optical tool for studying synaptic GABA_A receptor trafficking[J]. Journal of Cell Science, 2017, 130(22): 3933-3945.

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Please note: Our services can only be used for research purposes. Do not use in diagnostic or therapeutic procedures!