Human iPS Cells Differentiation Imaging Services
In the field of stem cells and regenerative medicine, scientists have differentiated iPS cells (iPSC) into a variety of cell types, including osteoblasts, chondrocytes, adipocytes, neuronal cells, cardiomyocytes, and vascular endothelial cells, by using targeted differentiation methods. These differentiated cells contribute to the understanding of disease mechanisms and the development of therapies. iPSC differentiation imaging allows real-time visualization and characterization of the differentiation process, and monitoring of morphological changes and functional features of differentiated iPSCs for disease modeling. In addition, iPSC differentiation imaging can also be used for potential drug response assessment, accurate drug screening, and the development of new therapeutic interventions.
Fig. 1 Directed differentiation of iPS cells (Amabile G and Meissner A, 2009).
Human iPS Cells Differentiation Imaging Services
CD BioSciences utilizes a variety of advanced microscopy technologies and high-resolution imaging equipment to provide customers with customized human iPSC cell differentiation imaging services based on customers' specific needs. Our services include but are not limited to:
- Evaluation of the status of human iPSC cells before and after differentiation.
- Acquisition of characteristic information including cell morphology, structure, and function.
- Real-time observation and quantitative analysis of the cell differentiation process and morphological changes using cell labeling and staining techniques. We utilize specific markers for analysis, such as:
- Neuron-specific β-III tubulin (TUJ1) expression indicates neuronal
- Pax6 indicates neural progenitor cells
- Oct-3/4 indicates undifferentiated iPSCs
- Human troponin T (cardiac) expression (cTNT1) indicates cardiomyocytes
- Albumin and HHNF-4α expression indicate hepatocytes
- Provision of detailed data reports for quantitative and qualitative analysis of imaging data.
Human iPS Cells Differentiation Imaging Workflow
Cell culture
Placement of induced pluripotent stem cells (iPSC) in appropriate culture medium and provide suitable conditions to promote its growth and proliferation.
Induced differentiation
Differentiation of induced pluripotent stem cells (iPSC) into target cell types by specific culture conditions, growth factors or chemicals.
Differentiation validation
Using appropriate techniques and methods to validate cell-specific markers during differentiation to ensure successful differentiation into the target cell type.
Imaging analysis
Observe and document the morphology, structure and function of differentiated cells using imaging techniques such as fluorescence microscopy, confocal microscopy and live cell imaging systems.
Data analysis
Perform quantitative and qualitative analysis of imaging results to interpret changes and characteristics during cell differentiation and generate data reports.
Deliverables
- Raw images
- Quantitative analysis data of human iPS cell differentiation
- Results analysis report
CD BioSciences has a professional team and advanced imaging equipment. The entire process of human iPS cells differentiation imaging is operated by experienced technicians to ensure the accuracy of the experiment. If you have any needs, please feel free to contact us, We can also customize the human iPS cell differentiation imaging service for you according to your research needs.
- Amabile G, Meissner A, Induced pluripotent stem cells: current progress and potential for regenerative medicine. Trends Mol Med, 2009, 15(2):59-68.
*If your organization requires the signing of a confidentiality agreement, please contact us by email.
Please note: Our services can only be used for research purposes. Do not use in diagnostic or therapeutic procedures!