Imaging Analysis for Drug Discovery & Development

Imaging in Ophthalmology Models

At CD BioSciences, we offer specialized services for the construction and imaging of ophthalmology models, utilizing both in vivo and in vitro modeling methods. Our expertise spans various disease models, including glaucoma, age-related macular degeneration (AMD), and diabetic retinopathy, coupled with advanced imaging techniques such as optical coherence tomography (OCT), adaptive optics scanning laser ophthalmoscopy (AOSLO), and fluorescence microscopy, to provide comprehensive data supporting preclinical ophthalmic research and drug development.

Roles of Imaging Technology in Preclinical Ophthalmology Research

Eye diseases represent a significant global health burden, encompassing a wide range of conditions affecting the visual system. Imaging technology plays a vital role in eye disease research, providing high-resolution visualization of ocular structures.

Imaging of the retina of wild-type mice as well as triple transgenic Alzheimer's disease mice (3xTg-AD). Figure 1. Retinal imaging of a wild type mouse and a triple transgenic mouse of Alzheimer's disease (3xTg-AD). (Batista A, et al. 2023)

What Ophthalmology Models Can We Provide Imaging Services for?

Corneal Disease Models
Ocular Inflammation and Uveitis Models
Lacrimal Disease Models

Lens Disease Models
Neuro-ophthalmology Models
Ocular Tumor Models

Glaucoma Models
Orbital Disease Models

What Can We Do for the Construction and Imaging of Ophthalmology Models?

Model Construction

Our disease modeling services provide researchers with customized in vivo and in vitro models of various eye diseases.

In vivo models: Employ specialized techniques to develop animal models that accurately mimic the key features of human disease, incorporating genetic modifications, environmental stressors, or pharmacological interventions as needed.
In vitro models: Utilize primary cell cultures (retinal cells, retinal pigment epithelial cells) and organoids to study specific cellular and molecular mechanisms underlying eye diseases.

Imaging and Analysis Services

Items	Service Detail
Imaging of Ocular Structure	Optical coherence tomography (OCT) for high-resolution 3D visualization and measurement of retinal layers. Micro-CT imaging for detailed assessment of anterior segment anatomy and optic nerve structure. Ultrasound biomicroscopy (UBM) to evaluate corneal and anterior chamber morphology. Magnetic resonance imaging (MRI) for non-invasive, high-contrast imaging of the whole eye.
Assessment of Retinal Function	Electroretinography (ERG) to evaluate rod and cone photoreceptor function. Adaptive optics scanning laser ophthalmoscopy (AOSLO) for single-cell level analysis of photoreceptors.
Visualization of Blood Vessels	Fluorescence angiography to image retinal and choroidal blood vessels Micro-CT angiography for high-resolution 3D mapping of the ocular vascular network Laser speckle contrast imaging to assess retinal blood flow dynamics
Cellular and Molecular Imaging	Confocal fluorescence microscopy to visualize cellular components and protein biomarkers. Multiphoton microscopy for deep tissue imaging of structural and functional changes.
Quantitative Data Analysis	Image Segmentation and Quantification: Precise delineation and measurement of retinal layers, blood vessels, cells, and other relevant structures. Morphometric Analysis: Quantitative assessment of structural changes, including retinal thickness, volume, and vascular density. Functional Analysis: Quantification of photoreceptor function and integrity based on AOSLO images. Vascular Analysis: Measurement of vascular parameters such as permeability, leakage, and perfusion. Cellular Analysis: Quantification of cell counts, morphology, and expression of specific markers.

Applications of Our Ophthalmology Model Construction and Imaging Services

Pathogenesis Studies: Investigating the progression of eye diseases and the cellular and molecular mechanisms underlying ocular disease pathogenesis.
Biomarker Discovery and Validation: Identifying potential biomarkers for diagnosis, prognosis, and treatment response.
Preclinical Drug Discovery and Development: Evaluating the efficacy and safety of novel drug candidates for various eye diseases.

From customized in vivo disease models to advanced imaging techniques like OCT, Fluorescence Microscopy, and MRI, CD BioSciences offers a complete solution to support your research and drug development programs for eye diseases. Our expertise in disease model construction and precise imaging ensures thorough analysis and accurate visualization of ocular diseases. Contact us today to discuss how our services can accelerate your research and development efforts in the field of ophthalmology.

Reference

Batista A, et al. Retinal imaging in animal models: Searching for biomarkers of neurodegeneration. Front Ophthalmol (Lausanne). 2023, 3:1156605.

*If your organization requires the signing of a confidentiality agreement, please contact us by email.

Please note: Our services can only be used for research purposes. Do not use in diagnostic or therapeutic procedures!

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