Epithelial tissue covers the surface of various organs inside and outside the human body, including the skin, respiratory tract, digestive tract, and urinary tract. The functions of epithelial tissues can be analyzed by using different imaging techniques such as transmission electron microscopy, high-resolution microscopy, and confocal microscopy. These imaging technologies can provide us with high-resolution image data to evaluate the barrier integrity, cellular connections, and molecular permeability of epithelial tissues, and quantify epithelial tissue growth dynamics to support disease research and drug discovery.

Fig. 1 Three types of epithelial tissue.

Imaging of Epithelial Tissue Functions

Barrier integrity assessment services

CD BioSciences provides barrier integrity assessment services to help clients evaluate the barrier integrity on epithelial tissues to facilitate research and drug discovery in respiratory diseases. Our services mainly include:

Traditional method assessment services

We use traditional trans-epithelial electrical resistance (TEER) and dextran permeability methods that can help clients assess the following properties of epithelial tissue:

➢ The integrity of the epithelial tissue barrier

➢ Degree of epithelial cell layer tight junctions

➢ Permeability of the epithelial barrier to macromolecules

High-content imaging analysis services

In addition to traditional methods, we utilize high-content imaging techniques combined with advanced image analysis software to help our clients capture high-resolution image data. Our service mainly includes:

➢ Assessing the characterization of focal connectivity complex structures in every plane of the image.

➢ Quantitative assessment of the expression and distribution of junction complex proteins.

3D pseudocomposite primary human bronchial epithelial cell model analysis

The 3D pseudocomposite primary human bronchial epithelial cell model is an in vitro cell culture model that mimics the properties and functions of human bronchial epithelial cells in a real physiological environment. We use this model to analyze epithelial barrier integrity, cell migration and cell differentiation for our clients.

Fig. 2 Non-keratinized stratified squamous epithelium of esophagus.

Post-infectious epithelial tissue imaging analysis services

CD BioSciences can use transmission electron microscopy to analyze the function of epithelial tissue, conduct detailed observation and analysis of epithelial tissue, and help customers understand the changes and degree of damage to epithelial tissue under infection. Our services include:

Epithelial tissue infection observation

Through transmission electron microscopy, we can observe and record epithelial tissue samples after infection, including morphological changes, cell shedding and necrosis of epithelial cells after infection.

Bacterial distribution analysis

We use transmission electron microscopy image analysis technology to locate and analyze bacterial distribution in epithelial tissue samples after infection, helping customers understand the interaction between bacteria and epithelial cells and the degree of infection.

Epithelial cell assessment analysis

Based on the transmission electron microscopy observation results, we can assess the degree of epithelial cell barrier disintegration and analyze cell detachment, necrosis, and damage to intercellular connections for customers. Provides the client with quantitative and qualitative information following the service regarding the extent of epithelial tissue damage.

Epithelial growing dynamics quantitative analysis services

CD BioSciences provides comprehensive quantitative analysis of epithelial tissue growth dynamics utilizing high temporal and cellular resolution imaging equipment. We can accurately quantify large-scale time-lapse image datasets to provide our clients with more comprehensive data analysis and results. Our services include, but are not limited to

Dynamic process capture

Through the advantage of utilizing in vitro culture and real-time imaging, we can capture the dynamic features and processes of epithelial tissue growth and morphogenesis, observe and analyze cellular changes at different time points and cell-cell interactions.

Quantitative description of epithelial cell dynamic behavior

We provide quantitative descriptions of the dynamic behavior of epithelial cells, including parameters such as cell shape changes, cell division direction changes. These descriptions help clients gain insight into the changes in cell behavior within the epithelial tissue.

CD BioSciences has a professional team and advanced imaging equipment. The whole process of imaging of epithelial tissue functions is operated by experienced technicians to ensure the accuracy of the experiment. If you have any needs, please feel free to contact us.

*If your organization requires the signing of a confidentiality agreement, please contact us by email.

Please note: Our services can only be used for research purposes. Do not use in diagnostic or therapeutic procedures!