Immunostaining Buffer for Hydrogel-Based Tissue Clearing Kit
This high lipid version tissue clearing kit is designed to support the CLARITY method of tissue clearing, offering a range of benefits for researchers. By enabling non-destructive tissue processing and facilitating 3D imaging, it empowers the examination of both normal and diseased soft tissues with exceptional precision. Specifically tailored for lipid clearing of soft tissues such as brain tissue, eye, and lymph nodes.
Information
Description:This reagent has been meticulously optimized for the precise immunostaining of soft tissues that have been cleared using the Hydrogel-Based Tissue Clearing Kit (TCKR001 and TCKR002). Following the process of lipid clearing, the resulting tissue-hydrogel hybrid exhibits exceptional transparency to light and remarkable permeability to molecules, thereby facilitating immunostaining and high-quality imaging.
Clearing Principle:Hydrogel-based tissue clearing
Kit Contents:Immunostaining buffer
Applications:This reagent has been meticulously optimized for the precise immunostaining of soft tissues that have been cleared using the Hydrogel-Based Tissue Clearing Kit (TCKR001 and TCKR002)
Form:Liquid
Technology:Hydrogel-based techniques employ two approaches: one involves utilizing monomer and initiator molecules to create a fabricated gel, while the other employs polyepoxide to produce a fortified tissue gel. These methods utilizing hydrogels offer several advantages, including the ability to preserve sufficient RNA quantities for assays like fluorescence in situ hybridization. Additionally, they facilitate significant tissue expansion due to the hydrogel mesh that securely binds the tissues together.
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