Each integrin consists of an α-subunit and a β-subunit to form a heterodimer. As adhesion receptors, integrins can play a very important role in a wide variety of biological functions by binding to extracellular matrix proteins or other adhesion receptors of neighboring cells. Currently, drugs targeting integrins have been extensively developed in many fields, including cardiovascular disease, inflammatory bowel disease, multiple sclerosis, and dry eye disease. Therefore, imaging analysis of integrin receptors is important for drug discovery.

Figure 1. Integrin heterodimer affinity and integrin activation (Sun G, et al. 2021).

Integrin Receptors Imaging Analysis

Epifluorescence microscopy and confocal laser scanning microscopy are common methods to study integrin receptor aggregation and distribution. However, the slow imaging speed and high phototoxicity limit its use in live-cell imaging. In recent years, with the development of imaging technology, fluorescence lifetime imaging microscopy (FLIM) has become a powerful tool for visual analysis of integrin receptor structure and function.

CD BioSciences has been committed to the development of fluorescent dyes and imaging technology for many years. We can quantify integrins in the in vivo environment by fluorescence lifetime imaging microscopy, fluorescence resonance energy transfer and fluorescence cross-correlation spectroscopy. We also can image interactions between integrin receptors and ligands. This will help to the development of drugs targeting integrin receptors.

Integrin Receptors Imaging Analysis Workflow

The following are the specific steps of integrin receptors imaging analysis

Delivery

Integrin heterodimer conformational changes
Specific activation of integrin heterodimers
Affinity of integrin heterodimers
Dynamics of integrin heterodimers
Other data that you need

Our Advantages

• Visualization of conformational changes of integrin receptors at the single-cell level
• High specificity, high sensitivity and quantitative measurement
• Experienced scientists provide experimental consultation
• Reasonable price and short turnaround time

CD BioSciences has a professional team and advanced imaging equipment. The entire process of integrin receptors imaging analysis is operated by experienced technicians to ensure the accuracy of the experiment. If you have any needs, please feel free to contact us.

1. Parsons M, Messent A J, Humphries J D, et al. Quantification of integrin receptor agonism by fluorescence lifetime imaging[J]. Journal of Cell Science, 2008, 121(Pt 3):265-271.
2. Sun G, Guillon E, Holley S A. Integrin intra-heterodimer affinity inversely correlates with integrin activatability[J]. Cell Reports, 2021, 35(10):109230.

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Please note: Our services can only be used for research purposes. Do not use in diagnostic or therapeutic procedures!