Metabolism includes a set of chemical reactions that occur in organisms. These reactions enable organisms to reproduce, develop, maintain their structure and respond to the environment. Intermediate metabolism describes all reactions related to the storage and production of metabolic energy required for the biosynthesis of low molecular weight compounds and energy storage compounds. In the intermediate metabolic pathway, the structure of each enzyme plays an important role in determining the specific properties of each reaction. Imaging analysis of intermediate metabolic process to study its metabolic mechanism is very important to maintain the normal operation of biological activities.

Figure 1. Classical pathways of intermediary metabolism (Ferreira, et al. 2020).

Intermediary Metabolism Imaging Analysis

Intermediate metabolism plays an important role in many physiological and pathological processes. In recent years, with the development of new fluorescent probes, it provides researchers with an effective tool for visual analysis of intermediate metabolism, so that they can study complex intermediate metabolism processes at the cellular level.

CD BioSciences is a biotechnology company, which has been committed to the research and development of fluorescent probes and live cell imaging technology for many years. We can provide you with fluorescent probes to visualize the process of intermediate metabolism and help you study the role and changes of different biomolecules in intermediate metabolism.

Intermediate Metabolism Imaging Analysis Workflow

The following are the specific steps of intermediate metabolism imaging analysis:

Delivery

¹H NMR, ¹³C NMR and mass spectra of fluorescent probes
Cytotoxicity of fluorescent probes
Spatial distribution of intermediates
Fluorescence images at different times
Provide other data according to your needs

Our Advantages

• Real-time monitoring of intermediate metabolism at the cell level
• High-throughput imaging analysis
• High signal-to-noise ratio, low cytotoxicity
• Experienced scientists provide experimental consultation
• Reasonable price and short turnaround time

CD BioSciences has a professional team and advanced imaging equipment. The entire process of intermediate imaging analysis is operated by experienced technicians to ensure the accuracy of the experiment. If you have any needs, please feel free to contact us. We will provide you with high-quality services that meet your needs.

1. Ferreira L M R, Li A M, Serafim T L, et al. Intermediary metabolism: An intricate network at the crossroads of cell fate and function[J]. Biochimica et Biophysica Acta (BBA)-Molecular Basis of Disease, 2020, 1866(10): 165887.

*If your organization requires the signing of a confidentiality agreement, please contact us by email.

Please note: Our services can only be used for research purposes. Do not use in diagnostic or therapeutic procedures!