Leukotrienes are products of arachidonic acid metabolism that play an important role in inflammatory processes. They are strong inflammatory factors that play an important role in pathological processes such as asthma, cystic fibrosis, hypertension, acute respiratory distress syndrome and ischemic heart disease. Its action is mediated by its two distinct receptors on the membrane, leukotriene receptor 1 and leukotriene receptor 2. Among them, leukotriene receptor 1 has high affinity with leukotrienes, and the role of leukotrienes in the process of inflammation is mainly achieved through leukotriene receptor 1. Therefore, leukotriene receptor 1 is an important target of anti-inflammatory and anti-allergic drugs, and imaging analysis of its structure is very important for the development of new anti-inflammatory and anti-allergic drugs.

Figure 1. Overall structure of the BLT1/Gi complex (Wang N, et al. 2022).

Leukotriene B4 Receptor1 Imaging Analysis

Leukotriene B4 receptor 1 is an important anti-inflammatory and antiallergic target. In recent years, there has been little progress in the development of anti-inflammatory and anti-allergic drugs targeting leukotriene B4 receptor 1, mainly due to the lack of the structure of activated leukotriene B4 receptor 1 as a design reference.

CD BioSciences has been committed to the development of imaging technology and fluorescent dye for many years. We can use cryo-electron microscopy to resolve the activation structure of leukotriene B4 receptor 1 bound to G protein. In addition, we can also use single-molecule localization microscopy to study the oligomerization state of leukotriene B4 receptor 1 at different cell cycles and locate individual molecules with nanometer precision. Our study will provide a theoretical basis for the design and synthesis of anti-inflammatory drugs targeting leukotriene B4 receptor 1.

Leukotriene B4 Receptor1 Imaging Analysis Workflow

The following are the specific steps of leukotriene B4 receptor1 imaging analysis

Delivery

The overall structure of BLT1/Gi complex
Quantitatively characterize the oligomeric state of leukotriene B4 receptor1
Percentage of leukotriene B4 receptor1 oligomer formed under agonistic and antagonistic conditions
Other data that you need

Our Advantages

• Visual analysis of leukotriene B4 receptor1
• Pinpoint the location of leukotriene B4 receptor1 in cells
• Experienced scientists provide experimental consultation
• Reasonable price and short turnaround time

CD BioSciences has a professional team and advanced imaging equipment. The entire process of leukotriene B4 receptor1 imaging analysis is operated by experienced technicians to ensure the accuracy of the experiment. If you have any needs, please feel free to contact us.  

1. Wang N, He X, Zhao J, et al. Structural basis of leukotriene B4 receptor 1 activation[J]. Nature Communications, 2022, 13(1): 1-10.
2. Joseph M D, Tomas Bort E, Grose R P, et al. Quantitative super-resolution imaging for the analysis of GPCR oligomerization[J]. Biomolecules, 2021, 11(10): 1503.

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Please note: Our services can only be used for research purposes. Do not use in diagnostic or therapeutic procedures!