Liver Fibrosis Imaging Analysis
Liver fibrosis is a common pathological pathway, which is caused by chronic inflammation of liver parenchyma and has nothing to do with the cause. An excess of fibrous collagen-rich and abnormal extracellular matrix (ECM) deposition are markers of liver fibrosis. Accurately assessing the degree of liver fibrosis is of great significance to the diagnosis and treatment of liver diseases.
Figure 1. Liver fibrosis.
Liver Fibrosis Imaging Analysis
Due to the differences in the diagnosis of liver disease between human and animal models, in vitro 3D liver models are increasingly used to develop therapies for liver fibrosis. CD BioSciences uses high-content, high-throughput imaging of collagen deposition and stellate cell activation to study the interaction of multiple cell subtypes in the fibrosis process to better understand the pathogenesis of liver fibrosis.
| Analysis method | High content imaging |
| Analysis instrument | High content imaging system |
| Cell models | HepaRG or primary human hepatocytes with NPCs Other/custom liver models available upon request |
| Fibrosis markers | Pan collagen, alpha-SMA, live/dead stain, DAPI |
| Sample requirements | 50µL of 20 mM solution or equivalent amount of solid |
Liver Fibrosis Imaging Analysis Workflow
3D cell cultures of mixed hepatocytes or hepatocyte-like cells and non-parenchymal cells are used for the determination of liver fibrosis, the detailed steps are as follows:
Step 1
The cells are cultured under standard culture conditions for 5-6 days to generate liver spheroids, which are aggregated to a size of approximately 200 μm in diameter.
Step 2
The sphere is treated with the test compound. If the test compound is intended to improve rather than prevent fibrosis, the test compound will be added after fibrosis is induced.
Step 3
After 1 hour of pretreatment (the specific time depends on the project requirements), add inducer for 48 hours.
Step 4
After treatment, the liver spheroids are labeled with vitality indicators, fixed, and labeled with antibodies against pan-collagen.
Step 5
The liver spheroid was cleared to make it transparent, and high-content imaging was performed on the orifice plate.
Step 6
The images are analyzed to quantify the fluorescence intensity and threshold area of collagen staining and viability.
Delivery
Dose response curves
EC50 values for cell viability, collagen deposition, and stellate cell activation in response to anti-fibrotic agents
Our Advantages
- Multiple liver fibrosis biomarkers can be used to evaluate the degree of liver fibrosis
- Multiple compounds can be screened in a single assay plate
- Experienced scientists provide experimental consultation
- Professional technicians provide you with high-quality personalized services
- Reduced workload and cost
CD BioSciences has a professional team and advanced imaging equipment. We provide you with liver fibrosis imaging analysis services by high-content imaging. According to your needs, we will design a personalized analysis plan for you to ensure a better completion of your project. If you have any needs for liver fibrosis imaging analysis, please feel free to contact us.
*If your organization requires the signing of a confidentiality agreement, please contact us by email.
Please note: Our services can only be used for research purposes. Do not use in diagnostic or therapeutic procedures!